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Título: | Nanobody-based blocking of binding ELISA for the detection of anti-NS1 Zika-Virus-Specific antibodies in convalescent patients |
Autor: | Delfin-Riela, Triana Rossotti, Martín A. Mattiuzzo, Giada Echaides, César González-Sapienza, Gualberto |
Tipo: | Artículo |
Descriptores: | SEROLOGIA, FLAVIVIRIDAE, INMUNOENSAYO, ANTICUERPOS, ZIKA |
Fecha de publicación: | 2023 |
Resumen: | Zika virus has spread around the world with rapid pace in the last five years. Although symptoms are typically mild and unspecific, Zika’s major impact occurs during pregnancy, generating a congenital syndrome. Serology plays a key role in its diagnosis. However, its use is limited due to the uncertainty caused by the cross-reaction of antibodies elicited in response to other flavivirus infections when tested in direct immunoassays. Using a panel of previously generated anti-Zika non-structural protein 1 (NS1) nanobodies, a set was selected that only recognizes epitopes present in Zika and is immunogenic to humans. A proper arrangement of these nanobodies was made and conditions were optimized in order to develop a novel serology assay. This new ELISA relies on the inhibition of the binding of a set of selected nanobodies to Zika-immobilized NS1 when previously incubated with Zika convalescent sera. Using the developed blocking of binding assay, it was possible to discriminate between Zika-specific and cross-reactive antibodies in serum samples from infections with Zika and other flaviviruses. |
Editorial: | MDPI |
EN: | Tropical Medicine and Infectious Disease, v.8, n° 1, 2023. -- e55 |
Citación: | Delfin-Riela, T, Rossotti, M, Mattiuzzo, G, y otros. "Nanobody-based blocking of binding ELISA for the detection of anti-NS1 Zika-Virus-Specific antibodies in convalescent patients". Tropical Medicine and Infectious Disease. [en línea] 2023 vol.8, n° 1, e55. DOI: https://doi.org/10.3390/tropicalmed8010055 |
Aparece en las colecciones: | Publicaciones académicas y científicas - Facultad de Química |
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AA Delfin-Riela, T. et al Tropical Medicine and Infectious Disease,v.8, n° 1, 2023. -- e55.pdf | 2,3 MB | Adobe PDF | Visualizar/Abrir |
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