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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Delfin-Riela, Triana | - |
dc.contributor.author | Rossotti, Martín A. | - |
dc.contributor.author | Mattiuzzo, Giada | - |
dc.contributor.author | Echaides, César | - |
dc.contributor.author | González-Sapienza, Gualberto | - |
dc.date.accessioned | 2023-11-21T11:54:28Z | - |
dc.date.available | 2023-11-21T11:54:28Z | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | Delfin-Riela, T, Rossotti, M, Mattiuzzo, G, y otros. "Nanobody-based blocking of binding ELISA for the detection of anti-NS1 Zika-Virus-Specific antibodies in convalescent patients". Tropical Medicine and Infectious Disease. [en línea] 2023 vol.8, n° 1, e55. DOI: https://doi.org/10.3390/tropicalmed8010055 | es |
dc.identifier.uri | https://hdl.handle.net/20.500.12008/41359 | - |
dc.description.abstract | Zika virus has spread around the world with rapid pace in the last five years. Although symptoms are typically mild and unspecific, Zika’s major impact occurs during pregnancy, generating a congenital syndrome. Serology plays a key role in its diagnosis. However, its use is limited due to the uncertainty caused by the cross-reaction of antibodies elicited in response to other flavivirus infections when tested in direct immunoassays. Using a panel of previously generated anti-Zika non-structural protein 1 (NS1) nanobodies, a set was selected that only recognizes epitopes present in Zika and is immunogenic to humans. A proper arrangement of these nanobodies was made and conditions were optimized in order to develop a novel serology assay. This new ELISA relies on the inhibition of the binding of a set of selected nanobodies to Zika-immobilized NS1 when previously incubated with Zika convalescent sera. Using the developed blocking of binding assay, it was possible to discriminate between Zika-specific and cross-reactive antibodies in serum samples from infections with Zika and other flaviviruses. | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | en | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | Tropical Medicine and Infectious Disease, v.8, n° 1, 2023. -- e55 | es |
dc.rights | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
dc.subject.other | SEROLOGIA | es |
dc.subject.other | FLAVIVIRIDAE | es |
dc.subject.other | INMUNOENSAYO | es |
dc.subject.other | ANTICUERPOS | es |
dc.subject.other | ZIKA | es |
dc.title | Nanobody-based blocking of binding ELISA for the detection of anti-NS1 Zika-Virus-Specific antibodies in convalescent patients | es |
dc.type | Artículo | es |
dc.contributor.filiacion | Delfin-Riela Triana, Universidad de la República (Uruguay). Facultad de Química, Instituto de Higiene, DEPBIO, Cátedra de Inmunología | - |
dc.contributor.filiacion | Rossotti Martín A., Universidad de la República (Uruguay). Facultad de Química, Instituto de Higiene, DEPBIO, Cátedra de Inmunología | - |
dc.contributor.filiacion | Mattiuzzo Giada, National Institute for Biological Standards and Control (NIBSC)-MHRA (Reino Unido). Division of Virology | - |
dc.contributor.filiacion | Echaides César, Intendencia Municial de Montevideo (Uruguay). Parque Lecoq | - |
dc.contributor.filiacion | González-Sapienza Gualberto, Universidad de la República (Uruguay). Facultad de Química, Instituto de Higiene, DEPBIO, Cátedra de Inmunología | - |
dc.rights.licence | Licencia Creative Commons Atribución (CC - By 4.0) | es |
dc.identifier.doi | https://doi.org/10.3390/tropicalmed8010055 | - |
Aparece en las colecciones: | Publicaciones académicas y científicas - Facultad de Química |
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AA Delfin-Riela, T. et al Tropical Medicine and Infectious Disease,v.8, n° 1, 2023. -- e55.pdf | 2,3 MB | Adobe PDF | Visualizar/Abrir |
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