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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Riera, Nadia | - |
dc.contributor.author | Davyt, Danilo | - |
dc.contributor.author | Durán, Rosario | - |
dc.contributor.author | Iraola, Gregorio | - |
dc.contributor.author | Lemanceau, Philippe | - |
dc.contributor.author | Bajsa, Natalia | - |
dc.date.accessioned | 2024-03-18T14:32:35Z | - |
dc.date.available | 2024-03-18T14:32:35Z | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | Riera, N., Davyt, D., Durán, R. y otros. "An antibiotic produced by Pseudomonas fluorescens CFBP2392 with antifungal activity against Rhizoctonia solani". Frontiers in Microbiology [en línea] 2023, v.14. -- e1286926 DOI: 10.3389/fmicb.2023.1286926 | es |
dc.identifier.uri | https://hdl.handle.net/20.500.12008/43131 | - |
dc.description.abstract | Pseudomonas fluorescens CFBP2392 has been recognized as a potential biocontrol agent due to its ability to suppress damping-off and root rot disease. This isolate has antibacterial activity in vitro as many other strains from the Pseudomonas fluorescens complex. In this work, the antibacterial and antifungal activity of the strain were explored. Dual culture assays evidenced the antifungal activity of the strain against different phytopathogens: Alternaria sp., Pythium ultimun, Fusarium oxysporum, and Rhizoctonia solani. Purification of an antifungal fraction was performed by preparative HPLC from the chemical extraction of growth media. The fraction showed altered R. solani growth and ultrastructure. Transmission electron microscopy revealed the purified compound induced hypertrophied mitochondria, membranous vesicles, and a higher number of vacuoles in R. salani cytoplasm. In addition, co-cultivation of P. fluorescens CFBP2392 with R. solani resulted in an enlarged and deformed cell wall. To gain genomic insights on this inhibition, the complete genome of P. fluorescens CFBP2392 was obtained with Oxford Nanopore technology. Different biosynthetic gene clusters (BGCs) involved in specialized metabolites production including a lokisin-like and a koreenceine-like cluster were identified. In accordance with the putative BGCs identified, sequence phylogeny analysis of the MacB transporter in the lokisin-like cluster further supports the similarity with other transporters from the amphisin family. Our results give insights into the cellular effects of the purified microbial metabolite in R. solani ultrastructure and provide a genomic background to further explore the specialized metabolite potential. | es |
dc.format.extent | 11 p. | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | en | es |
dc.publisher | Frontiers | es |
dc.relation.ispartof | Frontiers in Microbiology v.14, 2023. -- e1286926 | es |
dc.rights | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
dc.subject.other | ANTIFUNGICOS | es |
dc.subject.other | CONTROL BIOLOGICO | es |
dc.subject.other | ENFERMEDADES DE LAS PLANTAS | es |
dc.subject.other | GENOMICA | es |
dc.title | An antibiotic produced by Pseudomonas fluorescens CFBP2392 with antifungal activity against Rhizoctonia solani | es |
dc.type | Artículo | es |
dc.contributor.filiacion | Riera Nadia, Instituto de Investigaciones Biológicas Clemente Estable (Uruguay). Laboratorio de Ecología Microbiana.; Institut Pasteur de Montevideo (Uruguay). Laboratorio de Genómica Microbiana. | - |
dc.contributor.filiacion | Davyt Danilo, Universidad de la República (Uruguay). Facultad de Química. Laboratorio de Química Farmacéutica | - |
dc.contributor.filiacion | Durán Rosario, Institut Pasteur de Montevideo, Instituto de Investigaciones Biológicas Clemente Estable (Uruguay). Unidad Mixta de Bioquímica y Proteómica Analíticas. | - |
dc.contributor.filiacion | Iraola Gregorio, Institut Pasteur de Montevideo (Uruguay). Laboratorio de Genómica Microbiana. | - |
dc.contributor.filiacion | Lemanceau Philippe, Université de Bourgogne (Francia). Agroécologie, INRAE. Institut Agro. | - |
dc.contributor.filiacion | Bajsa Natalia, Instituto de Investigaciones Biológicas Clemente Estable (Uruguay). Laboratorio de Ecología Microbiana. | - |
dc.rights.licence | Licencia Creative Commons Atribución (CC - By 4.0) | es |
dc.identifier.doi | 10.3389/fmicb.2023.1286926 | - |
Aparece en las colecciones: | Publicaciones académicas y científicas - Facultad de Química |
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Riera, N. et al Frontiers in Microbiology.pdf | Artículo | 2,25 MB | Adobe PDF | Visualizar/Abrir |
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