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Título: | ISCOM‐matrices nanoformulation using the raw aqueous extract of Quillaja lancifolia (Q. brasiliensis) |
Autor: | Cibulski, Samuel Souza, Thalisson Amorim de Raimundo, Joanda Paola Nascimento, Yuri Mangueira Abreu, Lucas Silva Suarez, Norma Miraballes, Iris Roehe, Paulo Michael Araújo, Demetrius Antonio Machado de Tavares, Josean Fechine Silva, Marcelo Sobral da Silveira, Fernando |
Tipo: | Artículo |
Descriptores: | QUILLAJA BRASILIENSIS, SAPONINAS, VACUNAS, NANOTECNOLOGIA |
Fecha de publicación: | 2022 |
Resumen: | Quillaja saponins have an intrinsic capacity to interact with membrane lipids that self-assembles in nanoparticles (immunostimulating complexes or ISCOM-matrices) with outstanding immunoadjuvant activity and low toxicity profle. However, the expensive and laborious purifcation processes applied to purify Quillaja saponins used to assemble ISCOM-matrices show an important drawback in the large-scale use of this vaccine adjuvant. Thus, in this study, we describe a protocol to appropriately formulate ISCOM-matrices using the raw aqueous extract (AE) of Quillaja lancifolia leaves. In the presence of lipids, AE was able to self-assemble in nanostructures that resembles immunostimulating complexes (ISCOM). These negatively charged nanoparticles of approximately 40 nm were characterized by transmission electron microscopy and dynamic light scattering. In addition, well-known saponins with remarkable immunoadjuvant activity, as QS-21, were detected into nanoparticles. Thus, the easier, robust, cheaper, and environmentally friendly method developed here may be an alternative to the classical methods for ISCOM-matrices production that use high-purifed saponins. |
Editorial: | Springer |
EN: | BioNanoScience v. 12, 2022. -- pp. 1166–1171 |
Citación: | Cibulski, S, Souza, T, Raimundo, J, y otros. "ISCOM‐matrices nanoformulation using the raw aqueous extract of Quillaja lancifolia (Q. brasiliensis)". BioNanoScience. [en línea] 2022, vol. 12, pp. 1166–1171. DOI: https://doi.org/10.1007/s12668-022-01023-8 |
Aparece en las colecciones: | Publicaciones académicas y científicas - Facultad de Química |
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AA Cibulski, Samuel et al. BioNanoScience v.12, 2022. -- pp. 1166-1171.pdf | 1,04 MB | Adobe PDF | Visualizar/Abrir |
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