Por favor, use este identificador para citar o enlazar este ítem:
https://hdl.handle.net/20.500.12008/50547
Cómo citar
Registro completo de metadatos
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Umpiérrez, Ana | - |
dc.contributor.author | Ernst, Déborah | - |
dc.contributor.author | Cardozo, Andrea | - |
dc.contributor.author | Torres, Alexia | - |
dc.contributor.author | Fernández, Magalí | - |
dc.contributor.author | Fraga, Martín | - |
dc.contributor.author | Vignoli, Rafael | - |
dc.contributor.author | Bado, Inés | - |
dc.contributor.author | Vidal, Roberto | - |
dc.contributor.author | Zunino, Pablo | - |
dc.date.accessioned | 2025-07-10T17:25:56Z | - |
dc.date.available | 2025-07-10T17:25:56Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | UMPIÉRREZ, A., ERNST, D., CARDOZO, A., y otros. Non-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay. Austral. J. Vet. Sci [en línea] 2022, 54. DOI: 10.4067/S0719-81322022000200045 | es |
dc.identifier.uri | https://hdl.handle.net/20.500.12008/50547 | - |
dc.description.abstract | Shiga toxin-producing Escherichia coli (STEC) infections are responsible for acute illnesses and deaths in humans. Cattle and humans are exposed to STEC through faeces and contaminated food and water. The big six and O157 STEC serogroups are important food and water-borne human pathogens. Additionally, Stx1a, Stx2a and Stx2c subtypes are highly associated with the haemolytic uremic syndrome. This study aimed to determine Shiga toxin-subtypes, the presence of antigen 43 families, the genotypic and phenotypic antimicrobial susceptibility profiles, O-serogrouping, phylotypes and phylogenetic relatedness of STEC of calf origin. Sixteen STEC isolates from calf origin were analysed. PCR was performed to determine Stx subtypes, serogroups, the presence of ag43 I and II and phylotypes. The antimicrobial profile was evaluated and the presence of PMQR and fosfomycin genes was determined by PCR. The clonal relatedness of STEC was studied by PFGE. The genotypes stx1a+c, stx1a+, stx1a+/stx2e+, stx1a+c/stx2e and stx2a were detected. Ag43 II was the most prevalent among subfamilies. STEC isolates were serotyped as O103 (n=5) and O111 (n=6). Fifty per cent of the isolates were classified as B1 phylogroup, 4/16 as E, 1/16 as C, and 1/16 as F. Non-O157 STEC isolates showed a high level of diversity, independent of the geographical and farm-origin. Isolates were resistant to ampicillin, ciprofloxacin, gentamicin, and fosfomycin-trometamol. The gene fosA7 was detected in 1 isolate. The virulence profiles, including Shiga toxin-subtypes and serogroups, denote the potential harm of non-O157 STEC isolates to humans. We also confirmed that circulating non-O157 STEC from cattle present genetic heterogeneity and are susceptible to antibiotics. | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | en | es |
dc.relation.ispartof | Austral. J. Vet. Sci. 54, 2022 | es |
dc.rights | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
dc.subject | Non-O157 STEC | es |
dc.subject | Shiga toxin subtypes | es |
dc.subject | Antimicrobial resistance | es |
dc.title | Non-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay | es |
dc.type | Artículo | es |
dc.contributor.filiacion | Umpiérrez Ana, Instituto de Investigaciones Biológicas Clemente Estable (Uruguay). Departamento de Microbiología | - |
dc.contributor.filiacion | Ernst Déborah, Instituto de Investigaciones Biológicas Clemente Estable (Uruguay). Departamento de Microbiología | - |
dc.contributor.filiacion | Cardozo Andrea, Instituto de Investigaciones Biológicas Clemente Estable (Uruguay). Departamento de Microbiología | - |
dc.contributor.filiacion | Torres Alexia, Universidad de Chile (Chile). Facultad de Medicina. Instituto de Ciencias Biomédicas. Programa de Microbiología y Micología | - |
dc.contributor.filiacion | Fernández Magalí, Instituto de Investigaciones Biológicas Clemente Estable (Uruguay). Departamento de Microbiología | - |
dc.contributor.filiacion | Fraga Martín, Instituto Nacional de Investigación Agropecuaria (INIA) (Uruguay). Estación Experimental INIA La Estanzuela. Plataforma de Investigación en Salud Animal | - |
dc.contributor.filiacion | Vignoli Rafael, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y Virología | - |
dc.contributor.filiacion | Bado Inés, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y Virología | - |
dc.contributor.filiacion | Vidal Roberto, Universidad de Chile (Chile). Facultad de Medicina. Instituto de Ciencias Biomédicas. Programa de Microbiología y Micología | - |
dc.contributor.filiacion | Zunino Pablo, Instituto de Investigaciones Biológicas Clemente Estable (Uruguay). Departamento de Microbiología | - |
dc.rights.licence | Licencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0) | es |
dc.identifier.doi | 10.4067/S0719-81322022000200045 | - |
Aparece en las colecciones: | Artículos - Instituto de Higiene |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | ||
---|---|---|---|---|---|
Non-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay.pdf | Artículo original | 267,8 kB | Adobe PDF | Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons