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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | González, Sabina | - |
dc.contributor.author | Geymonat, Juan Pablo | - |
dc.contributor.author | Hernández, Elba | - |
dc.contributor.author | Marqués, Juan Martín | - |
dc.contributor.author | Schelotto, Felipe | - |
dc.contributor.author | Varela, Gustavo | - |
dc.date.accessioned | 2024-02-23T14:42:13Z | - |
dc.date.available | 2024-02-23T14:42:13Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | GONZALEZ, S., GEYMONAT, JP., HERNANDEZ, E., y otros. Usefulness of real-time PCR assay targeting lipL32 gene for diagnosis of human leptospirosis in Uruguay. J Infect Dev Ctries [en línea] 7(12), 2013. Doi: 10.3855/jidc.4110 | es |
dc.identifier.uri | https://hdl.handle.net/20.500.12008/42614 | - |
dc.description.abstract | Introduction: Assays based on DNA amplification can provide information that contributes to the initial management of patients with leptospirosis. However, these have not been adopted in Uruguay. Our aim was to evaluate the performance of the lipL32 real-time PCR (qPCR) for diagnosis of leptospirosis. Methodology: We analyzed by microscopic agglutination test (MAT) and lipL32 qPCR serum samples from 183 patients with suspected leptospirosis. To establish the analytical sensitivity of the qPCR, experimentally spiked samples with known amounts of Leptospira interrogans were analyzed. Results: The analytical sensitivity of the qPCR was 102 leptospires/mL. In 98 patients MAT results were negative meanwhile 85 showed positive reactions, revealing acute infections. Twenty six acute-phase sera of these 85 patients showed a positive signal by qPCR (diagnostic sensitivity 30%). In these patients the average time between onset of symptoms and collection of the first sample was 8 days. In patients with negative results for qPCR and positive MAT results (n=59) the average interval between onset of symptoms and collection of the first sample was 13 days. The qPCR did not yield false positive results. Conclusions: The qPCR had a lower diagnostic sensitivity than MAT and a higher cost. However, it allowed to make an early diagnosis in 26 patients. In patients with confirmed acute infections and negative results by qPCR, more than 8 days had elapsed between the onset of the illness and extraction of the first serum sample. Our data support that the qPCR from sera have clinical utility within the first week of illness. | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | en | es |
dc.relation.ispartof | J Infect Dev Ctries. 7(12), 2013 | es |
dc.rights | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
dc.subject | LipL32 qPCR | es |
dc.subject | MAT | es |
dc.subject | Leptospirosis | es |
dc.subject | Laboratory diagnosis | es |
dc.subject | Uruguay | es |
dc.title | Usefulness of real-time PCR assay targeting lipL32 gene for diagnosis of human leptospirosis in Uruguay | es |
dc.type | Artículo | es |
dc.contributor.filiacion | González Sabina, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y Virología | - |
dc.contributor.filiacion | Geymonat Juan Pablo, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y Virología | - |
dc.contributor.filiacion | Hernández Elba, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y Virología | - |
dc.contributor.filiacion | Marqués Juan Martín, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Desarrollo Biotecnológico | - |
dc.contributor.filiacion | Schelotto Felipe, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y Virología | - |
dc.contributor.filiacion | Varela Gustavo, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Bacteriología y Virología | - |
dc.rights.licence | Licencia Creative Commons Atribución (CC - By 4.0) | es |
dc.identifier.doi | 10.3855/jidc.4110 | - |
Aparece en las colecciones: | Artículos - Instituto de Higiene |
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Usefulness of real-time PCR assay targeting lipL32 gene.pdf | Artículo original | 467,27 kB | Adobe PDF | Visualizar/Abrir |
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