Por favor, use este identificador para citar o enlazar este ítem:
https://hdl.handle.net/20.500.12008/42513
Cómo citar
Registro completo de metadatos
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Cancela, Florencia | - |
dc.contributor.author | Ramos, Natalia | - |
dc.contributor.author | Smyth, Davida S | - |
dc.contributor.author | Etchebehere, Claudia | - |
dc.contributor.author | Berois, Mabel | - |
dc.contributor.author | Rodríguez, Jesica | - |
dc.contributor.author | Rufo, Caterina | - |
dc.contributor.author | Alemán, Alicia | - |
dc.contributor.author | Borzacconi, Liliana | - |
dc.contributor.author | López, Julieta | - |
dc.contributor.author | González, Elizabeth | - |
dc.contributor.author | Botto, Germán | - |
dc.contributor.author | Thornhill, Starla G | - |
dc.contributor.author | Mirazo, Santiago | - |
dc.contributor.author | Trujillo, Mónica | - |
dc.date.accessioned | 2024-02-20T12:23:56Z | - |
dc.date.available | 2024-02-20T12:23:56Z | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | CANCELA, F., RAMOS, N., SMYTH, DS., y otros. Wastewater surveillance of SARS-CoV-2 genomic populations on a country-wide scale through targeted sequencing. PLoS One [en línea] 18, 2023. DOI: 10.1371/journal.pone.0284483 | es |
dc.identifier.uri | https://hdl.handle.net/20.500.12008/42513 | - |
dc.description.abstract | SARS-CoV-2 surveillance of viral populations in wastewater samples is recognized as a useful tool for monitoring epidemic waves and boosting health preparedness. Next generation sequencing of viral RNA isolated from wastewater is a convenient and cost-effective strategy to understand the molecular epidemiology of SARS-CoV-2 and provide insights on the population dynamics of viral variants at the community level. However, in low- and middle-income countries, isolated groups have performed wastewater monitoring and data has not been extensively shared in the scientific community. Here we report the results of monitoring the co-circulation and abundance of variants of concern (VOCs) of SARS-CoV-2 in Uruguay, a small country in Latin America, between November 2020—July 2021 using wastewater surveillance. RNA isolated from wastewater was characterized by targeted sequencing of the Receptor Binding Domain region within the spike gene. Two computational approaches were used to track the viral variants. The results of the wastewater analysis showed the transition in the overall predominance of viral variants in wastewater from No-VOCs to successive VOCs, in agreement with clinical surveillance from sequencing of nasal swabs. The mutations K417T, E484K and N501Y, that characterize the Gamma VOC, were detected as early as December 2020, several weeks before the first clinical case was reported. Interestingly, a non-synonymous mutation described in the Delta VOC, L452R, was detected at a very low frequency since April 2021 when using a recently described sequence analysis tool (SAM Refiner). Wastewater NGS-based surveillance of SARS-CoV-2 is a reliable and complementary tool for monitoring the introduction and prevalence of VOCs at a community level allowing early public health decisions. This approach allows the tracking of symptomatic and asymptomatic individuals, who are generally under-reported in countries with limited clinical testing capacity. Our results suggests that wastewater-based epidemiology can contribute to improving public health responses in low- and middle-income countries. | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | en | es |
dc.relation.ispartof | PLoS One. 18, 2023 | es |
dc.rights | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
dc.subject | Aguas Residuales | es |
dc.subject | SARS-CoV-2 | es |
dc.title | Wastewater surveillance of SARS-CoV-2 genomic populations on a country-wide scale through targeted sequencing | es |
dc.type | Artículo | es |
dc.contributor.filiacion | Cancela Florencia, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica | - |
dc.contributor.filiacion | Ramos Natalia, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica | - |
dc.contributor.filiacion | Smyth Davida S, Texas A&M University-San Antonio (Estados Unidos). Department of Life Sciences | - |
dc.contributor.filiacion | Etchebehere Claudia, Ministerio de Educación y Cultura (Uruguay). Instituto de Investigaciones Biológicas Clemente Estable. Departamento de Bioquímica y Genómica Microbiana | - |
dc.contributor.filiacion | Berois Mabel, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica | - |
dc.contributor.filiacion | Rodríguez Jesica, Universidad de la República (Uruguay). Facultad de Química. Polo Tecnológico de Pando. Laboratorio de Alimentos y Nutrición | - |
dc.contributor.filiacion | Rufo Caterina, Universidad de la República (Uruguay). Facultad de Química. Polo Tecnológico de Pando. Laboratorio de Alimentos y Nutrición | - |
dc.contributor.filiacion | Alemán Alicia, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica de Medicina Preventiva | - |
dc.contributor.filiacion | Borzacconi Liliana, Universidad de la República (Uruguay). Facultad de Ingeniería. Instituto de Ingeniería Química | - |
dc.contributor.filiacion | López Julieta, Universidad de la República (Uruguay). Facultad de Ingeniería. Departamento de Ingeniería Ambiental | - |
dc.contributor.filiacion | González Elizabeth, Universidad de la República (Uruguay). Facultad de Ingeniería. Departamento de Ingeniería Ambiental | - |
dc.contributor.filiacion | Botto Germán, Universidad de la República (Uruguay). Facultad de Medicina. Departamento de Métodos Cuantitativos | - |
dc.contributor.filiacion | Thornhill Starla G, Texas A&M University-San Antonio (Estados Unidos). Department of Life Sciences | - |
dc.contributor.filiacion | Mirazo Santiago, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica de Bacteriología y Virología | - |
dc.contributor.filiacion | Trujillo Mónica, Queensborough Community College of The City University of New York (Estados Unidos). Department of Biological Sciences and Geology | - |
dc.rights.licence | Licencia Creative Commons Atribución (CC - By 4.0) | es |
dc.identifier.doi | 10.1371/journal.pone.0284483 | - |
Aparece en las colecciones: | Artículos - Instituto de Higiene |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | ||
---|---|---|---|---|---|
Wastewater surveillance of SARS-CoV-2.pdf | Artículo original | 1,83 MB | Adobe PDF | Visualizar/Abrir |
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons