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Título: | Albendazole specifically disrupts microtubules and protein turnover in the tegument of the cestode Mesocestoides corti |
Autor: | Guarnaschelli, Inés Koziol, Uriel |
Tipo: | Artículo |
Palabras clave: | Microtubules, Larvae, Protein synthesis, Benzimidazoles, Tegument proteins, Tubulins, Cestodes, Flatworms |
Fecha de publicación: | 2025 |
Resumen: | Parasitic flatworms, such as cestodes and trematodes, are covered by a syncytial tissue known as the tegument. It consists of a superficial band of cytoplasm (the distal tegument) that is connected by cytoplasmic bridges to multiple cell bodies (cytons) that lay beneath the basal lamina and contain the nuclei. We characterized the cytoskeleton of the tegument of the model cestode Mesocestoides corti and determined the effects of albendazole and albendazole sulfoxide on its organization. These anthelmintics are known to target beta-tubulin in helminths, and their effects have been extensively studied in nematodes, but the specific cells and tissues that are affected are not well understood in parasitic flatworms. Using antibodies that detect different tubulin subunits and post-translational modifications, we show that microtubules in the distal tegument have a unique organization, with bouquets of microtubules radiating from the cytoplasmic bridges, suggesting a role in intracellular traffic. In contrast, actin filaments were largely absent from the distal tegument. The microtubules of the tegument were specifically sensitive to low, chemotherapeutically relevant concentrations of albendazole and albendazole sulfoxide. This was correlated with the accumulation of secretory material in the cytons, and low concentrations of albendazole strongly reduced the incorporation of newly synthesized proteins in the distal tegument, as determined by metabolic labeling. Unexpectedly, albendazole also induced a global decrease in protein synthesis, which was independent of the activation of the unfolded protein response. Our work identifies the tegument as a sensitive target of benzimidazoles in cestodes and indicates that translational inhibition may contribute to the anthelmintic effect of benzimidazoles. |
Editorial: | PLoS ONE |
EN: | PLoS Pathogens, 2025, 21(6): e1013221. |
Financiadores: | ANII: FCE_1_2019_1_156125 |
Citación: | Guarnaschelli, I y Koziol, U. "Albendazole specifically disrupts microtubules and protein turnover in the tegument of the cestode Mesocestoides corti". PLoS Pathogens. [en línea] 2025, 21(6): e1013221. 24 h. DOI: 10.1371/journal.ppat.1013221 |
ISSN: | 1553-7374 |
Licencia: | Licencia Creative Commons Atribución (CC - By 4.0) |
Aparece en las colecciones: | Publicaciones académicas y científicas - Facultad de Ciencias |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | ||
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10.1371-journal.ppat.1013221.pdf | 2,08 MB | Adobe PDF | Visualizar/Abrir |
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