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Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12008/49627 How to cite
Title: Uncovering a multitude of stage-specific splice variants and putative protein isoforms generated along mouse spermatogenesis
Authors: Romeo, Carlos
Trovero Martínez, María Fernanda
Radío, Santiago
Smircich, Pablo
Rodríguez-Casuriaga, Rosana
Geisinger Wschebor, Adriana
Sotelo Silveira, José Roberto
Type: Artículo
Keywords: Spermatogenesis, Transcriptome, Alternative splicing, lncRNAs, Testi
Issue Date: 2024
Abstract: Background: Mammalian testis is a highly complex and heterogeneous tissue. This complexity, which mostly derives from spermatogenic cells, is reflected at the transcriptional level, with the largest number of tissue-specific genes and long noncoding RNAs (lncRNAs) compared to other tissues, and one of the highest rates of alternative splicing. Although it is known that adequate alternative-splicing patterns and stage-specific isoforms are critical for successful spermatogenesis, so far only a very limited number of reports have addressed a detailed study of alternative splicing and isoforms along the different spermatogenic stages. Results: In the present work, using highly purified stage-specific testicular cell populations, we detected 33,002 transcripts expressed throughout mouse spermatogenesis not annotated so far. These include both splice variants of already annotated genes, and of hitherto unannotated genes. Using conservative criteria, we uncovered 13,471 spermatogenic lncRNAs, which reflects the still incomplete annotation of lncRNAs. A distinctive feature of lncRNAs was their lower number of splice variants compared to protein-coding ones, adding to the conclusion that lncRNAs are, in general, less complex than mRNAs. Besides, we identified 2,794 unannotated transcripts with high coding potential (including some arising from yet unannotated genes), many of which encode unnoticed putative testisspecific proteins. Some of the most interesting coding splice variants were chosen, and validated through RT-PCR. Remarkably, the largest number of stage-specific unannotated transcripts are expressed during early meiotic prophase stages, whose study has been scarcely addressed in former transcriptomic analyses. Conclusions: We detected a high number of yet unannotated genes and alternatively spliced transcripts along mouse spermatogenesis, hence showing that the transcriptomic diversity of the testis is considerably higher than previously reported. This is especially prominent for specific, underrepresented stages such as those of early meiotic prophase, and its unveiling may constitute a step towards the understanding of their key events.
Description: Datos disponibles en: https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA548952
Publisher: Springer
IN: BMC Genomics, 2024, 25: 295.
Sponsors: ANII: FCE_1_2021_1_166510
Citation: Romeo, C, Trovero Martínez, M, Radío, S [y otros autores]. "Uncovering a multitude of stage-specific splice variants and putative protein isoforms generated along mouse spermatogenesis". BMC Genomics. [en línea] 2024, 25: 295. 17 h. DOI: 10.1186/s12864-024-10170-z
ISSN: 1471-2164
License: Licencia Creative Commons Atribución (CC - By 4.0)
Appears in Collections:Publicaciones académicas y científicas - Facultad de Ciencias

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