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Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12008/41422 How to cite
Title: T908 polymeric micelles improved the uptake of Sgc8-c aptamer probe in tumor-bearing mice: a co-association study between the probe and preformed nanostructures
Authors: Castelli Pedriel, María Romina
Ibarra, Manuel
Faccio, Ricardo
Miraballes, Iris
Fernández Lomonaco, Marcelo Luis
Moglioni, Albertina
Cabral González, Pablo
Cerecetto, Hugo
Glisoni, Romina J.
Calzada, Victoria
Type: Artículo
Keywords: Sgc8-c aptame, probe, Polymeric micelles, Liposomes, Active targeting
Issue Date: 2022
Abstract: Aptamers are oligonucleotides that have the characteristic of recognizing a target with high affinity and specificity. Based on our previous studies, the aptamer probe Sgc8-c-Alexa647 is a promising tool for molecular imaging of PTK7, which is an interesting biomarker in cancer. In order to improve the delivery of this probe as well as create a novel drug delivery nanosystem targeted to the PTK7 receptor, we evaluate the co-association between the probe and preformed nanostructures. In this work, preformed pegylated liposomes (PPL) and linear and branched pristine polymeric micelles (PMs), based on PEO–PPO–PEO triblock copolymers were used: poloxamer F127® and poloxamines T1307® and T908®. For it, Sgc8-c-Alexa647 and its co-association with the different nanostructures was exhaustively analyzed. DLS analysis showed nanometric sizes, and TEM and AFM showed notable differences between free- and co-associated probe. Likewise, all nanosystems were evaluated on A20 lymphoma cell line overexpressing PTK7, and the confocal microscopy images showed distinctness in cellular uptake. Finally, the biodistribution in BALB/c mice bearing lymphoma-tumor and pharmacokinetic study revealed an encouraging profile for T908-probe. All data obtained from this work suggested that PMs and, more specifically T908 ones, are good candidates to improve the pharmacokinetics and the tumor uptake of aptamer-based probes.
Publisher: MDPI
IN: Pharmaceuticals, 2022, 15(1): 15.
DOI: 10.3390/ph15010015
ISSN: 1424-8247
Citation: Castelli Pedriel, M, Ibarra, M, Faccio, R, [y otros autores]. "T908 polymeric micelles improved the uptake of Sgc8-c aptamer probe in tumor-bearing mice: a co-association study between the probe and preformed nanostructures". Pharmaceuticals. [en línea] 2022, 15(1): 15. 20 h. DOI: 10.3390/ph15010015
License: Licencia Creative Commons Atribución (CC - By 4.0)
Appears in Collections:Publicaciones académicas y científicas - Facultad de Ciencias

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