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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Fontenla Martínez, Santiago | - |
dc.contributor.author | Langleib de Souza, Mauricio | - |
dc.contributor.author | Torre-Escudero, Eduardo de la | - |
dc.contributor.author | Domínguez, María Fernanda | - |
dc.contributor.author | Robinson, Mark W. | - |
dc.contributor.author | Tort, José F. | - |
dc.date.accessioned | 2023-11-22T14:17:33Z | - |
dc.date.available | 2023-11-22T14:17:33Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Fontenla Martínez, S, Langleib de Souza, M, Torre-Escudero, E, [y otros autores]. "Role of fasciola hepatica small RNAs in the interaction with the mammalian host". Frontiers in Cellular and Infection Microbiology. [en línea] 2022, 11: 812141. 18 h. DOI: 10.3389/fcimb.2021.812141 | es |
dc.identifier.issn | 2235-2988 | - |
dc.identifier.uri | https://hdl.handle.net/20.500.12008/41416 | - |
dc.description | Material complementario: https://www.frontiersin.org/articles/10.3389/fcimb.2021.812141/full#supplementary-material | es |
dc.description.abstract | MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression being involved in many different biological processes and play a key role in developmental timing. Additionally, recent studies have shown that miRNAs released from parasites are capable of regulating the expression of host genes. In the present work, we studied the expression patterns of ncRNAs of various intra-mammalian life-cycle stages of the liver fluke, Fasciola hepatica, as well as those packaged into extracellular vesicles and shed by the adult fluke. The miRNA expression profile of the intra-mammalian stages shows important variations, despite a set of predominant miRNAs that are highly expressed across all stages. No substantial variations in miRNA expression between dormant and activated metacercariae were detected, suggesting that they might not be central players in regulating fluke gene expression during this crucial step in the invasion of the definitive host. We generated a curated pipeline for the prediction of putative target genes that reports only sites conserved between three different prediction approaches. This pipeline was tested against an iso-seq curated database of the 3’ UTR regions of F. hepatica genes to detect miRNA regulation networks within liver fluke. Several functions related to the host immune response or modulation were enriched among the targets of the most highly expressed parasite miRNAs, stressing that they might be key players during the establishment and maintenance of infection. Additionally, we detected fragments derived from the processing of tRNAs, in all developmental stages analyzed, and documented the presence of novel long tRNA fragments enriched in vesicles. We confirmed the presence of at least 5 putative vault RNAs (vtRNAs), that are expressed across different stages and enriched in vesicles. The presence of tRNA fragments and vtRNAs in vesicles raise the possibility that they could be involved in the host-parasite interaction. | es |
dc.description.sponsorship | CSIC: I+D 2019_521 | es |
dc.format.extent | 18 h. | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | en | es |
dc.publisher | Frontiers Media | es |
dc.relation.ispartof | Frontiers in Cellular and Infection Microbiology, 2022, 11: 812141 | es |
dc.rights | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
dc.subject | Fasciola hepatica | es |
dc.subject | Micro RNA (miRNA) | es |
dc.subject | Development | es |
dc.subject | tRNA | es |
dc.subject | Vault RNA (vtRNA) | es |
dc.subject | Extracellular vesicles (EVs) | es |
dc.subject | Host-parasite | es |
dc.title | Role of fasciola hepatica small RNAs in the interaction with the mammalian host | es |
dc.type | Artículo | es |
dc.contributor.filiacion | Fontenla Martínez Santiago, Universidad de la República (Uruguay). Facultad de Medicina. | - |
dc.contributor.filiacion | Langleib de Souza Mauricio, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. | - |
dc.contributor.filiacion | Torre-Escudero Eduardo de la | - |
dc.contributor.filiacion | Domínguez María Fernanda, Universidad de la República (Uruguay). Facultad de Medicina. | - |
dc.contributor.filiacion | Robinson Mark W. | - |
dc.contributor.filiacion | Tort José F., Universidad de la República (Uruguay). Facultad de Medicina. | - |
dc.rights.licence | Licencia Creative Commons Atribución (CC - By 4.0) | es |
dc.identifier.doi | 10.3389/fcimb.2021.812141 | - |
Aparece en las colecciones: | Publicaciones académicas y científicas - Facultad de Ciencias |
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10.3389fcimb.2021.812141.pdf | 7,03 MB | Adobe PDF | Visualizar/Abrir |
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