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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Alonso, Julia R. | - |
dc.contributor.author | Silva, Alejandro | - |
dc.contributor.author | Fernández, Ariel | - |
dc.contributor.author | Arocena, Miguel | - |
dc.date.accessioned | 2023-09-01T13:53:30Z | - |
dc.date.available | 2023-09-01T13:53:30Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Alonso, J, Silva, A, Fernández, A y otros. "Computational multifocus fluorescence microscopy for three-dimensional visualization of multicellular tumor spheroids". Journal of Biomedical Optics. [en línea] 2022 junio 2022, 27 (6). 10.1117/1.JBO.27.6.066501 | es |
dc.identifier.uri | https://hdl.handle.net/20.500.12008/39757 | - |
dc.description.abstract | Significance: Three-dimensional (3D) visualization of multicellular tumor spheroids (MCTS) in fluorescence microscopy can rapidly provide qualitative morphological information about the architecture of these cellular aggregates, which can recapitulate key aspects of their in vivo counterpart. Aim: The present work is aimed at overcoming the shallow depth-of-field (DoF) limitation in fluorescence microscopy while achieving 3D visualization of thick biological samples under study. Approach: A custom-built fluorescence microscope with an electrically focus-tunable lens was developed to optically sweep in-depth the structure of MCTS. Acquired multifocus stacks were combined by means of postprocessing algorithms performed in the Fourier domain. Results: Images with relevant characteristics as extended DoF, stereoscopic pairs as well as reconstructed viewpoints of MCTS were obtained without segmentation of the focused regions or estimation of the depth map. The reconstructed images allowed us to observe the 3D morphology of cell aggregates. Conclusions: Computational multifocus fluorescence microscopy can provide 3D visualization in MCTS. This tool is a promising development in assessing the morphological structure of different cellular aggregates while preserving a robust yet simple optical setup. | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | en | es |
dc.relation.ispartof | Journal of Biomedical Optics, junio 2022, 27 (6) | es |
dc.rights | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
dc.subject | fluorescence microscopy | es |
dc.subject | three-dimensional visualization | es |
dc.subject | stereoscopic pairs | es |
dc.subject | computational optical imaging | es |
dc.subject.other | Imagenología Tridimensional | es |
dc.subject.other | Neoplasias | es |
dc.subject.other | Algoritmos | es |
dc.subject.other | Humanos | es |
dc.subject.other | Imagenología Tridimensional/métodos | es |
dc.subject.other | Microscopía Fluorescente/métodos | es |
dc.subject.other | Neoplasias/diagnóstico por imagen | es |
dc.subject.other | Esferoides Celulares | es |
dc.title | Computational multifocus fluorescence microscopy for three-dimensional visualization of multicellular tumor spheroids | es |
dc.type | Artículo | es |
dc.contributor.filiacion | Alonso Julia R., Universidad de la República. Facultad de Ingeniería. Instituto de Física | - |
dc.contributor.filiacion | Silva Alejandro, Universidad de la República. Facultad de Ingeniería. Instituto de Física | - |
dc.contributor.filiacion | Fernández Ariel, Universidad de la República. Facultad de Ingeniería. Instituto de Física | - |
dc.contributor.filiacion | Arocena Miguel, Instituto de Investigaciones Biológicas Clemente Estable. Departamento de Genómica, Universidad de la República. Facultad de Odontología. Cátedra de Bioquímica y Biofísica | - |
dc.rights.licence | Licencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0) | es |
dc.identifier.doi | 10.1117/1.JBO.27.6.066501 | - |
Aparece en las colecciones: | Publicaciones académicas y científicas 2020- - Facultad de Odontología |
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Computational multifocus fluorescence microscopy for three dimensional visualization of multicellular tumor spheroids.pdf | 3,6 MB | Adobe PDF | Visualizar/Abrir |
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