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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Vázquez Alberdi, Lucía | - |
dc.contributor.author | Rosso, Gonzalo | - |
dc.contributor.author | Veloz, Lucía | - |
dc.contributor.author | Romero, Carlos | - |
dc.contributor.author | Farías, Joaquina | - |
dc.contributor.author | Di Tomaso, María Vittoria | - |
dc.contributor.author | Calero, Miguel | - |
dc.contributor.author | Kun González, Alejandra E. | - |
dc.date.accessioned | 2023-07-21T17:26:50Z | - |
dc.date.available | 2023-07-21T17:26:50Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Vázquez Alberdi, L, Rosso, G, Veloz, L, [y otros autores]. "Curcumin and ethanol effects in Trembler-J Schwann cell culture". Biomolecules. [en línea] 2022,12(4): 515.19 h. DOI: 10.3390/biom12040515 | es |
dc.identifier.issn | 2218273X | - |
dc.identifier.uri | https://hdl.handle.net/20.500.12008/38336 | - |
dc.description.abstract | Charcot-Marie-Tooth (CMT) syndrome is the most common progressive human motor and sensory peripheral neuropathy. CMT type 1E is a demyelinating neuropathy affecting Schwann cells due to peripheral-myelin-protein-22 (PMP22) mutations, modelized by Trembler-J mice. Curcumin, a natural polyphenol compound obtained from turmeric (Curcuma longa), exhibits dose- and time-varying antitumor, antioxidant and neuroprotective properties, however, the neurotherapeutic actions of curcumin remain elusive. Here, we propose curcumin as a possible natural treatment capable of enhancing cellular detoxification mechanisms, resulting in an improvement of the neurodegenerative Trembler-J phenotype. Using a refined method for obtaining enriched Schwann cell cultures, we evaluated the neurotherapeutic action of low dose curcumin treatment on the PMP22 expression, and on the chaperones and autophagy/mammalian target of rapamycin (mTOR) pathways in Trembler-J and wild-type genotypes. In wild-type Schwann cells, the action of curcumin resulted in strong stimulation of the chaperone and macroautophagy pathway, whereas the modulation of ribophagy showed a mild effect. However, despite the promising neuroprotective effects for the treatment of neurological diseases, we demonstrate that the action of curcumin in Trembler-J Schwann cells could be impaired due to the irreversible impact of ethanol used as a common curcumin vehicle necessary for administration. These results contribute to expanding our still limited understanding of PMP22 biology in neurobiology and expose the intrinsic lability of the neurodegenerative Trembler-J genotype. Furthermore, they unravel interesting physiological mechanisms of cellular resilience relevant to the pharmacological treatment of the neurodegenerative Tremble J phenotype with curcumin and ethanol. We conclude that the analysis of the effects of the vehicle itself is an essential and inescapable step to comprehensibly assess the effects and full potential of curcumin treatment for therapeutic purposes. | es |
dc.description.sponsorship | ANNI:FCE_1_2019_1_155539 | es |
dc.format.extent | 19 h. | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | en_US | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | Biomolecules, 2022,12(4): 515. | es |
dc.rights | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
dc.subject | CMT1E | es |
dc.subject | Trembler-J. | es |
dc.subject | Curcumin | es |
dc.subject | Ethanol | es |
dc.subject | Hsps | es |
dc.subject | Autophagy | es |
dc.title | Curcumin and ethanol effects in Trembler-J Schwann cell culture | es |
dc.type | Artículo | es |
dc.contributor.filiacion | Vázquez Alberdi Lucía, IIBCE | - |
dc.contributor.filiacion | Rosso Gonzalo | - |
dc.contributor.filiacion | Veloz Lucía, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. | - |
dc.contributor.filiacion | Romero Carlos, IIBCE | - |
dc.contributor.filiacion | Farías Joaquina, Universidad de la República (Uruguay). Centro Universitario de Tacuarembó, CENUR Noreste. | - |
dc.contributor.filiacion | Di Tomaso María Vittoria, IIBCE | - |
dc.contributor.filiacion | Calero Miguel | - |
dc.contributor.filiacion | Kun González Alejandra E., Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. | - |
dc.rights.licence | Licencia Creative Commons Atribución (CC - By 4.0) | es |
dc.identifier.doi | 10.3390/biom12040515 | - |
Aparece en las colecciones: | Publicaciones académicas y científicas - Facultad de Ciencias |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | ||
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10.3390biom12040515.pdf | 25,85 MB | Adobe PDF | Visualizar/Abrir |
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