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Title: | A deletion in SARS-CoV- 2 ORF7 identified in COVID-19 outbreak in Uruguay |
Authors: | Panzera Crespo, Yanina Ramos, Natalia Frabasile Giurato, Sandra Alicia Calleros Basilio, Lucía Marandino, Ana Tomás Custodio, Gonzalo Martín Techera, Claudia Grecco Patiño, Sofía Fuques Villalba, Eddie Goñi Mazzitelli, Natalia Ramas, Vivivana Coppola, Leticia Chiparelli, Héctor Sorhouet, Cecilia Mogdasy, Cristina Arbiza, Juan Delfraro Vázquez, Adriana Beatriz Pérez Crossa, Ruben Gustavo |
Type: | Artículo |
Keywords: | SARS-CoV- 2, ORF7a |
Issue Date: | 2021 |
Abstract: | The analysis of genetic diversity in SARS-CoV-2 is the focus of several studies, providing insights into how the virus emerged and evolves. Most common changes in SARS-CoV-2 are single or point nucleotide substitutions; meanwhile, insertions and deletions (indels) have been identified as a less frequent source of viral genetic variability. Here, we report the emergence of a 12-nucleotide deletion in ORF7a, resulting in a 4-amino acid in-frame deletion. The Δ12 variant was identified in viruses from patients of a single outbreak and represents the first report of this deletion in South American isolates. Phylogenetic analysis revealed that Δ12 strains belong to the lineage B.1.1 and clustered separated from the remaining Uruguayan strains. The ∆12 variant was detected in 14 patients of this outbreak by NGS sequencing and/or two rapid and economic methodologies: Sanger amplicon sequencing and capillary electrophoresis. The presence of strong molecular markers as the deletion described here are useful for tracking outbreaks and reveal a significant aspect of the SARS-CoV-2 evolution on the robustness of the virus to keep its functionality regardless loss of genetic material. |
Publisher: | Willey |
IN: | Transboundary and Emerging Diseases, 2021, 68(6): 3075-3082. |
DOI: | 10.1111/tbed.14002 |
ISSN: | 1865-1682 |
Citation: | Panzera Crespo, Y, Ramos, N, Frabasile Giurato, S [y otros autores]. "A deletion in SARS-CoV- 2 ORF7 identified in COVID-19 outbreak in Uruguay". Transboundary and Emerging Diseases. [en línea] 2021, 68(6): 3075-3082. 8 h. DOI: 10.1111/tbed.14002. |
License: | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
Appears in Collections: | Publicaciones académicas y científicas - Facultad de Ciencias |
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