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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Fagúndez, Pablo | - |
dc.contributor.author | Botasini, Santiago | - |
dc.contributor.author | Tosar Rovira, Juan Pablo | - |
dc.contributor.author | Méndez, Eduardo | - |
dc.date.accessioned | 2022-08-19T13:08:18Z | - |
dc.date.available | 2022-08-19T13:08:18Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Fagúndez, P, Botasini, S, Tosar Rovira, J [y otro autor]. "Systematic process evaluation of the conjugation of proteins to gold nanoparticles". Heliyon. [en línea] 2021, 7(6): e07392. 10 h. Doi: 10.1016/j.heliyon.2021.e07392. | es |
dc.identifier.issn | 2405-8440 | - |
dc.identifier.uri | https://hdl.handle.net/20.500.12008/33231 | - |
dc.description.abstract | The present work addresses some fundamental aspects in the preparation of protein-conjugated gold nanoparticles, in order to ensure an appropriate final product. Ten broadly available and/or easy to implement analytical tools were benchmarked and compared in their capacity to provide reliable and conclusive information for each step of the procedure. These techniques included transmission electron microscopy, UV/VIS spectroscopy, dynamic light scattering, zeta-potential, Fourier-transformed infrared spectroscopy, colloidal stability titration, end-point colloidal stability analysis, cyclic voltammetry, agarose gel electrophoresis and size-exclusion chromatography (SEC). Four different proteins widely used as adaptors or blocking agents were tested, together with 13 nm gold nanoparticles containing different surface chemistries. Among all tested techniques, some of the least popular among nanomaterial scientists probed to be the most informative, including colloidal stability, gel electrophoresis and SEC; the latter being also an efficient purification procedure. These three techniques provide low-cost, low time consuming, sensitive and robust ways to assess the success of the nanoparticle bioconjugation steps, especially when used in adequate combinations. | es |
dc.description.sponsorship | CSIC: C-164-348 | es |
dc.description.sponsorship | ANII: POS_NAC_2016_1_130350 | es |
dc.format.extent | 10 h | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | en | es |
dc.publisher | Cell press | es |
dc.relation.ispartof | Heliyon, 2021, 7(6): e07392 | es |
dc.rights | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
dc.subject | Bioconjugation | es |
dc.subject | Colloidal stability | es |
dc.subject | Gold nanoparticles | es |
dc.subject | Covalent attachment | es |
dc.subject | Non-covalent attachment | es |
dc.title | Systematic process evaluation of the conjugation of proteins to gold nanoparticles | es |
dc.type | Artículo | es |
dc.contributor.filiacion | Fagúndez Pablo, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares. | - |
dc.contributor.filiacion | Botasini Santiago, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica. | - |
dc.contributor.filiacion | Tosar Rovira Juan Pablo, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares. | - |
dc.contributor.filiacion | Méndez Eduardo, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica. | - |
dc.rights.licence | Licencia Creative Commons Atribución (CC - By 4.0) | es |
dc.identifier.doi | 10.1016/j.heliyon.2021.e07392 | - |
Aparece en las colecciones: | Publicaciones académicas y científicas - Facultad de Ciencias |
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101016jheliyon2021e07392.pdf | 1,13 MB | Adobe PDF | Visualizar/Abrir |
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