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dc.contributor.authorTosar Rovira, Juan Pablo-
dc.contributor.authorArab, Tanina-
dc.contributor.authorMallick, Emily R.-
dc.contributor.authorHuang, Yiyao-
dc.contributor.authorDong, Liang-
dc.contributor.authorLiao, Zhaohao-
dc.identifier.citationTosar Rovira, J, Arab, T, Mallick, E [y otros autores]. "Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single-particle analysis platforms". Journal of Extracellular Vesicles. [en línea] 2021, 10(6): e12079. 20 h. Doi: 10.1002/jev2.12079.es
dc.descriptionIngreso parcial de los coautores.es
dc.description.abstractWe compared four orthogonal technologies for sizing, counting, and phenotyping of extracellular vesicles (EVs) and synthetic particles. The platforms were: singleparticle interferometric reflectance imaging sensing (SP-IRIS) with fluorescence, nanoparticle tracking analysis (NTA) with fluorescence, microfluidic resistive pulse sensing (MRPS), and nanoflow cytometry measurement (NFCM). EVs from the human T lymphocyte line H9 (high CD81, low CD63) and the promonocytic line U937 (low CD81, high CD63) were separated from culture conditioned medium (CCM) by differential ultracentrifugation (dUC) or a combination of ultrafiltration (UF) and size exclusion chromatography (SEC) and characterized by transmission electron microscopy (TEM) and Western blot (WB). Mixtures of synthetic particles.(silica and polystyrene spheres) with known sizes and/or concentrations were also tested.MRPS andNFCMreturned similar particle counts,whileNTAdetected counts approximately one order of magnitude lower for EVs, but not for synthetic particles. SP-IRIS events could not be used to estimate particle concentrations. For sizing, SPIRIS, MRPS, and NFCM returned similar size profiles, with smaller sizes predominating (per power law distribution), but with sensitivity typically dropping off below diameters of 60 nm. NTA detected a population of particles with a mode diameter greater than 100 nm. Additionally, SP-IRIS, MRPS, and NFCM were able to identify at least three of four distinct size populations in a mixture of silica or polystyrene nanoparticles. Finally, for tetraspanin phenotyping, the SP-IRIS platform in fluorescencemode was able to detect at least twomarkers on the same particle, while NFCM detected either CD81 or CD63. Based on the results of this study, we can draw conclusions about existing single-particle analysis capabilities that may be useful for EV biomarker development and mechanistic studies.es
dc.format.extent20 hes
dc.publisherInternational Society for Extracellular Vesicleses
dc.relation.ispartofJournal of Extracellular Vesicles, 2021, 10(6): e12079.es
dc.rightsLas obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)es
dc.subjectExtracellular vesicleses
dc.subjectNanoflow cytometryes
dc.subjectNanoparticle tracking analysises
dc.subjectResistive pulse sensinges
dc.subjectSingle particle interferometric reflectance imaging sensinges
dc.titleCharacterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single-particle analysis platformses
dc.contributor.filiacionTosar Rovira Juan Pablo, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares.-
dc.contributor.filiacionArab Tanina-
dc.contributor.filiacionMallick Emily R.-
dc.contributor.filiacionHuang Yiyao-
dc.contributor.filiacionDong Liang-
dc.contributor.filiacionLiao Zhaohao-
dc.rights.licenceLicencia Creative Commons Atribución (CC - By 4.0)es
Appears in Collections:Publicaciones académicas y científicas - Facultad de Ciencias

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