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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Casaravilla, Cecilia | - |
dc.contributor.author | Pittini, Álvaro | - |
dc.contributor.author | Rückerl, Dominik | - |
dc.contributor.author | Allen, Judith E. | - |
dc.contributor.author | Díaz, Álvaro | - |
dc.contributor.editor | De’Broski, R. Herbert | - |
dc.date.accessioned | 2022-05-25T15:26:56Z | - |
dc.date.available | 2022-05-25T15:26:56Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Casaravilla, C, Pittini, Á, Rückerl, [y otros] "Activation of the NLRP3 inflammasome by particles from the Echinococcus granulosus laminated ayer". Infection and Immunity. [en línea] 2020, 88(9): e00190-20. 13 h. DOI: 10.1128/IAI.00190-20 | es |
dc.identifier.issn | 1098-5522 | - |
dc.identifier.uri | https://hdl.handle.net/20.500.12008/31665 | - |
dc.description.abstract | The interaction of dendritic cells and macrophages with a variety ofrigid noncellular particles triggers activation of the NLRP3 inflammasome and conse-quent secretion of interleukin 1 (IL-1 ). Noncellular particles can also be generatedin the context of helminth infection, since these large pathogens often shed theiroutermost structures during growth and/or molting. One such structure is the mas-sive, mucin-based, soft, flexible laminated layer (LL), which protects the larval stagesof cestodes of the genusEchinococcus. We show that particles from theEchinococcusgranulosusLL (pLL) trigger NLRP3- and caspase-1-dependent IL-1 in lipopolysaccha-ride (LPS)-primed mouse bone marrow-derived dendritic cells (BMDC). This responsecan be elicited by pLL too large for phagocytosis and nonetheless requires actin dy-namics, Syk, and phosphatidylinositol 3-kinase (PI3K). These three requirements hadalready been observed in our previous study on the alteration by pLL of CD86,CD40, IL-10, and IL-12 responses to LPS in BMDC; however, we now show that thesealterations are independent of NLRP3 and caspase-1. In other words, an initial inter-action with particles requiring actin dynamics, Syk, and PI3K, but not phagocytosis,elicits both NLRP3-dependent and NLRP3-independent responses. Intraperitoneal in-jection of pLL induced IL-1 , suggesting that contact with LL materials induces IL-1 in theE. granulosusinfection setting. Our results extend our understanding of NLRP3inflammasome activation by noncellular particulate materials both to helminth-derived materials and to flexible/soft materials. | es |
dc.format.extent | 13 h. | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | en | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Infection and Immunity, 2020, 88(9): e00190-20 | es |
dc.rights | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
dc.subject | Echinococcus | es |
dc.subject | Laminated layer | es |
dc.subject | Alum | es |
dc.subject | NLRP3 | es |
dc.subject | PI3K | es |
dc.subject | Membrane affinity-triggered signaling | es |
dc.subject | Adjuvants | es |
dc.subject | Dendritic cells | es |
dc.subject | Exophagy | es |
dc.subject | Helminths | es |
dc.subject | Inflammation | es |
dc.subject | Macrophages | es |
dc.subject | Mucin | es |
dc.title | Activation of the NLRP3 inflammasome by particles from the Echinococcus granulosus laminated layer | es |
dc.type | Artículo | es |
dc.contributor.filiacion | Casaravilla Cecilia, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica | - |
dc.contributor.filiacion | Pittini Álvaro, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica | - |
dc.contributor.filiacion | Rückerl D. | - |
dc.contributor.filiacion | Allen J. E. | - |
dc.contributor.filiacion | Díaz Álvaro, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica | - |
dc.rights.licence | Licencia Creative Commons Atribución (CC - By 4.0) | es |
dc.identifier.doi | 10.1128/IAI.00190-20 | - |
Aparece en las colecciones: | Publicaciones académicas y científicas - Facultad de Ciencias |
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Fichero | Descripción | Tamaño | Formato | ||
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10.1128IAI.00190-20.pdf | 1,79 MB | Adobe PDF | Visualizar/Abrir |
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