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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Díaz Viraqué, Florencia | - |
dc.contributor.author | Pita Mimbacas, Sebastián | - |
dc.contributor.author | Greif, Gonzalo | - |
dc.contributor.author | Souza, R. | - |
dc.contributor.author | Iraola, Gregorio | - |
dc.contributor.author | Robello Porto, Carlos | - |
dc.contributor.editor | Ochman, Howard | - |
dc.date.accessioned | 2021-05-26T14:06:07Z | - |
dc.date.available | 2021-05-26T14:06:07Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Díaz Viraqué, F, Pita Mimbacas, S, Greif, G, y otros "Nanopore sequencing significantly improves genome assembly of the protozoan parasite Trypanosoma cruzi". Genome Biology and Evolution. [en línea] 2019, 11(7): 1952-1957. 6 h. DOI: 10.1093/gbe/evz129 | es |
dc.identifier.issn | 1759-6653 | - |
dc.identifier.uri | https://hdl.handle.net/20.500.12008/27890 | - |
dc.description.abstract | Chagas disease was described by Carlos Chagas, who first identified the parasite Trypanosoma cruzi from a 2-year-old girl called Berenice. Many T. cruzi sequencing projects based on short reads have demonstrated that genome assembly and downstream comparative analyses are extremely challenging in this species, given that half of its genome is composed of repetitive sequences. Here, we report de novo assemblies, annotation, and comparative analyses of the Berenice strain using a combination of Illumina short reads andMinION long reads. Our work demonstrates that Nanopore sequencing improves T. cruzi assembly contiguity and increases the assembly size in 16Mb. Specifically, we found that assembly improvement also refines the completeness of coding regions for both single-copy genes and repetitive transposable elements. Beyond its historical and epidemiological importance, Berenice constitutes a fundamental resource because it now constitutes a high-quality assembly available for TcII (clade C), a prevalent lineage causing human infections in South America. The availability of Berenice genome expands the known genetic diversity of these parasites and reinforces the idea that T. cruzi is intraspecifically divided in three main clades. Finally, this work represents the introduction of Nanopore technology to resolve complex protozoan genomes, supporting its subsequent application for improving trypanosomatid and other highly repetitive genomes. | es |
dc.format.extent | 6 h. | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | en | es |
dc.publisher | Society for Molecular Biology and Evolution | es |
dc.relation.ispartof | Genome Biology and Evolution, 2019, 11(7): 1952-1957 | es |
dc.rights | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
dc.subject | Trypanosoma cruzi | es |
dc.subject | Berenice | es |
dc.subject | Hybrid assembly | es |
dc.subject | Protozoan parasites | es |
dc.subject | Chagas disease | es |
dc.subject | Oxford Nanopore Technologies | es |
dc.title | Nanopore sequencing significantly improves genome assembly of the protozoan parasite Trypanosoma cruzi | es |
dc.type | Artículo | es |
dc.contributor.filiacion | Díaz Viraqué Florencia, Instituto Pasteur (Montevideo). | - |
dc.contributor.filiacion | Pita Mimbacas Sebastián, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología | - |
dc.contributor.filiacion | Greif Gonzalo, Instituto Pasteur (Montevideo). | - |
dc.contributor.filiacion | Souza R. | - |
dc.contributor.filiacion | Iraola Gregorio, Instituto Pasteur (Montevideo). | - |
dc.contributor.filiacion | Robello Porto Carlos, Universidad de la República (Uruguay). Facultad de Medicina. | - |
dc.rights.licence | Licencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0) | es |
dc.identifier.doi | 10.1093/gbe/evz129 | - |
Aparece en las colecciones: | Publicaciones académicas y científicas - Facultad de Ciencias |
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10.1093gbeevz129.pdf | 414,83 kB | Adobe PDF | Visualizar/Abrir |
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