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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Pita Mimbacas, Sebastián | - |
dc.contributor.author | Díaz Viraqué, Florencia | - |
dc.contributor.author | Iraola, Gregorio | - |
dc.contributor.author | Robello Porto, Carlos | - |
dc.contributor.editor | Beltran, Esther | - |
dc.date.accessioned | 2021-05-11T16:55:43Z | - |
dc.date.available | 2021-05-11T16:55:43Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Pita Mimbacas, S, Díaz Viraqué, F, Iraola, G, y otro "The tritryps comparative repeatome: insights on repetitive reement evolution in trypanosomatid pathogens". Genome Biology and Evolution. [en línea] 2019, 11(2): 546–551. 6 h. DOI: 10.1093/gbe/evz017 | es |
dc.identifier.issn | 1759-6653 | - |
dc.identifier.uri | https://hdl.handle.net/20.500.12008/27638 | - |
dc.description.abstract | The major human pathogens Trypanosoma cruzi, Trypanosoma brucei, and Leishmania major are collectively known as the Tritryps. The initial comparative analysis of their genomes has uncovered that Tritryps share a great number of genes, but repetitive DNA seems to be extremely variable between them. However, the in-depth characterization of repetitive DNA in these pathogens has been in part neglected, mainly due to the well-known technical challenges of studying repetitive sequences from de novo assemblies using short reads. Here, we compared the repetitive DNA repertories between the Tritryps genomes using genome-wide, low-coverage Illumina sequencing coupled to RepeatExplorer analysis. Our work demonstrates that this extensively implemented approach for studying higher eukaryote repeatomes is also useful for protozoan parasites like trypanosomatids, as we recovered previously observed differences in the presence and amount of repetitive DNA families. Additionally, our estimations of repetitive DNA abundance were comparable to those obtained from enhanced-quality assemblies using longer reads. Importantly, our methodology allowed us to describe a previously undescribed transposable element in Leishmania major (TATE element), highlighting its potential to accurately recover distinctive features from poorly characterized repeatomes. Together, our results support the application of this low-cost, low-coverage sequencing approach for the extensive characterization of repetitive DNA evolutionary dynamics in trypanosomatid and other protozoan genomes. | es |
dc.format.extent | 6 h. | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | en | es |
dc.publisher | Society for Molecular Biology and Evolution | es |
dc.relation.ispartof | Genome Biology and Evolution, 2019, 11(2): 546–551 | es |
dc.rights | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
dc.subject | Trypanosomatids | es |
dc.subject | Tritryps | es |
dc.subject | Repetitive DNA | es |
dc.subject | RepeatExplorer | es |
dc.subject | Transposable elements | es |
dc.title | The tritryps comparative repeatome: insights on repetitive element evolution in trypanosomatid pathogens | es |
dc.type | Artículo | es |
dc.contributor.filiacion | Pita Mimbacas Sebastián, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología | - |
dc.contributor.filiacion | Díaz Viraqué Florencia, Instituto Pasteur (Montevideo). | - |
dc.contributor.filiacion | Iraola Gregorio, Instituto Pasteur (Montevideo). | - |
dc.contributor.filiacion | Robello Carlos, Universidad de la República (Uruguay). Facultad de Medicina | - |
dc.rights.licence | Licencia Creative Commons Atribución (CC - By 4.0) | es |
dc.identifier.doi | 10.1093/gbe/evz017 | - |
Aparece en las colecciones: | Publicaciones académicas y científicas - Facultad de Ciencias |
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10.1093gbeevz017.pdf | 308,35 kB | Adobe PDF | Visualizar/Abrir |
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