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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Berná, Luisa | - |
dc.contributor.author | Chiribao, María Laura | - |
dc.contributor.author | Greif, Gonzalo | - |
dc.contributor.author | Rodríguez Esquibel, Matías Daniel | - |
dc.contributor.author | Álvarez-Valín, Fernando | - |
dc.contributor.author | Robello Porto, Carlos | - |
dc.date.accessioned | 2019-11-29T15:55:24Z | - |
dc.date.available | 2019-11-29T15:55:24Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Berná, L., Chiribao, M.L., Greif, G., y otros. "Transcriptomic analysis reveals metabolic switches and surface remodeling as key processes for stage transition in Trypanosoma cruz". PeerJ [en línea]. 2017 (3) e3017. doi: 10.7717/peerj.3017 | es |
dc.identifier.issn | 2167-8359 | - |
dc.identifier.uri | https://hdl.handle.net/20.500.12008/22603 | - |
dc.description.abstract | American trypanosomiasis is a chronic and endemic disease which affects millions of people. Trypanosoma cruzi, its causative agent, has a life cycle that involves complex morphological and functional transitions, as well as a variety of environmental conditions. This requires a tight regulation of gene expression, which is achieved mainly by post-transcriptional regulation. In this work we conducted an RNAseq analysis of the three major life cycle stages of T. cruzi, amastigotes, epimastigotes and trypomastigotes. This analysis allowed us to delineate specific transcriptomic profiling for each stage, and also to identify those biological processes of major relevance in each state. Stage specific expression profiling evidenced the plasticity of T. cruzi to adapt quickly to different conditions, with particular focus on membrane remodeling and metabolic shifts along the life cycle. Epimastigotes, which replicate in the gut of insect vector, showed higher expression of genes related to energy metabolism, mainly Krebs cycle, respiratory chain and oxidative phosphorylation related genes, and anabolism related genes associated to nucleotide and steroid biosynthesis; also a general down-regulation of surface glycoproteins was seen at this stage. Trypomastigotes, living extracellularly in the bloodstream of mammals, express a plethora of surface proteins and signaling genes involved in invasion and evasion of immune response. Amastigotes mostly express membrane transporters and genes involved in regulation of cell cycle, an also express a specific subset of surface glycoproteins coding genes. In addition, these results allowed us to improve the annotation of Dm28c genome, identifying new ORFs and set the stage for construction of networks of co-expression, which can give clues about coded proteins of unknown functions | es |
dc.format.extent | 32 h | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | en | es |
dc.publisher | PeerJ Inc | es |
dc.relation.ispartof | PeerJ, 2017, (3) e3017 | es |
dc.rights | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
dc.subject | Chagas disease | es |
dc.subject | Metabolic regulation | es |
dc.subject | Trypanosoma cruzi | es |
dc.subject | Surface proteins | es |
dc.subject | RNA-seq | es |
dc.title | Transcriptomic analysis reveals metabolic switches and surface remodeling as key processes for stage transition in Trypanosoma cruzi | es |
dc.type | Artículo | es |
dc.contributor.filiacion | Berná Luisa, Instituto Pasteur (Montevideo) | - |
dc.contributor.filiacion | Chiribao María Laura, Instituto Pasteur (Montevideo) | - |
dc.contributor.filiacion | Greif Gonzalo, Instituto Pasteur (Montevideo) | - |
dc.contributor.filiacion | Rodríguez Esquibel Matías Daniel, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología | - |
dc.contributor.filiacion | Alvarez-Valín Fernando, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología | - |
dc.contributor.filiacion | Robello Porto Carlos, Instituto Pasteur (Montevideo) | - |
dc.rights.licence | Licencia Creative Commons Atribución (CC - By 4.0) | es |
dc.identifier.doi | 10.7717/peerj.3017 | - |
Aparece en las colecciones: | Publicaciones académicas y científicas - Facultad de Ciencias |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | ||
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107717peerj3017.pdf | 1,09 MB | Adobe PDF | Visualizar/Abrir |
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