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Título: | Comparative repeatome analysis on Triatoma infestans Andean and Non-Andean lineages, main vector of Chagas disease |
Autor: | Pita Mimbacas, Sebastián Panzera Arballo, Francisco Mora, Pablo Vela, J. Cuadrado, Ángeles Sánchez, Antonio Palomeque, Teresa Lorite, Pedro |
Tipo: | Artículo |
Palabras clave: | Chagas Disease, Trypanosoma Cruzi, Congenital transmission |
Fecha de publicación: | 2017 |
Resumen: | Triatoma infestans is the most important Chagas disease vector in South America. Two main evolutionary lineages, named Andean and non-Andean, have been recognized by geographical distribution, phenetic and genetic characteristics. One of the main differences is the genomic size, varying over 30% in their haploid DNA content. Here we realize a genome wide analysis to compare the repetitive genome fraction (repeatome) between both lineages in order to identify the main repetitive DNA changes occurred during T. infestans differentiation process. RepeatExplorer analysis using Illumina reads showed that both lineages exhibit the same amount of non-repeat sequences, and that satellite DNA is by far the major component of repetitive DNA and the main responsible for the genome size differentiation between both lineages. We characterize 42 satellite DNA families, which are virtually all present in both lineages but with different amount in each lineage. Furthermore, chromosomal location of satellite DNA by fluorescence in situ hybridization showed that genomic variations in T. infestans are mainly due to satellite DNA families located on the heterochromatic regions. The results also show that many satDNA families are located on the euchromatic regions of the chromosomes. |
Editorial: | PLoS |
EN: | PloS one, 2017 12 (7), p. e0181635 |
DOI: | 10.1371/journal.pone.0181635 |
ISSN: | 1932-6203 |
Citación: | Pita, S., Panzera, F., Mora, P. [y otros autores]. "Comparative repeatome analysis on Triatoma infestans Andean and Non-Andean lineages, main vector of Chagas disease". PloS one [en línea]. 2017 12 (7), p. e0181635. doi: 10.1371/journal.pone.0181635 |
Licencia: | Licencia Creative Commons Atribución (CC - By 4.0) |
Aparece en las colecciones: | Publicaciones académicas y científicas - Facultad de Ciencias |
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101371journalpone0181635.pdf | 21,9 MB | Adobe PDF | Visualizar/Abrir |
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