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Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12008/22432 How to cite
Title: Genomic characterization and seroprevalence studies on alphaviruses in Uruguay
Authors: Burgueño, Analía
Frabasile Giurato, Sandra Alicia
Díaz, L. A.
Cabrera Castro, Andrés M.
Pisano, María Belén
Rivarola, M. E.
Contigiani, M.
Delfraro Vázquez, Adriana Beatriz
Type: Artículo
Keywords: Alphaviruses, Virus, Genomics
Issue Date: 2018
Abstract: Alphaviruses (Togaviridae) are arboviruses frequently associated with emerging infectious diseases. In this study, we aimed to investigate the presence of alphaviruses in Uruguay by detecting the viral genome in mosquitoes and neutralizing antibodies in equines. A total of 3,575 mosquitoes were analyzed for alphavirus genome detection. Serologic studies were performed on 425 horse sera by plaque reduction neutralization test (PRNT80) against Venezuelan equine encephalitis virus (VEEV) subtype IAB, Pixuna virus (PIXV), Rio Negro virus (RNV), western equine encephalitis virus (WEEV), and Madariaga virus (MADV). Mosquitoes belonging to six genera were captured and 82.9% were identified as Culex pipiens. Two Cx. pipiens pools collected in Fray Bentos and Las Toscas localities were alphavirus positive, and phylogenetic analyses showed that the sequences grouped into two different clusters: the lineage I of eastern equine encephalitis virus and RNV (VEEV complex), respectively. Plaque reduction neutralization test assays showed antibodies against strains of the VEEV complex, MADV, and WEEV. Rio Negro virus was the most geographically widespread virus, showing higher seroprevalences (up to 20%). Seroprevalences against VEEV IAB ranged between 4.6% and 13%; antibodies against PIXV, WEEV, and MADV were less frequent (3–4%). In conclusion, RNV exhibited the highest seroprevalence in horses, a wide geographical distribution, and viral genome was detected in Cx. pipiens mosquitoes. Madariaga virus had a low seroprevalence in equines, but an epizootic lineage typical of North America was detected in Cx. pipiens mosquitoes. Taken together, our results show that alphaviruses are present in Uruguay with variable occurrence and geographical distribution being a potential threat for human and equine health.
Publisher: American Society of Tropical Medicine and Hygiene
IN: American Journal of Tropical Medicine and Hygiene, 2018, 98 (6), 1811-1818
Citation: Burgueño, A., Frabasile Giurato, S., Díaz, L. y otros. "Genomic characterization and seroprevalence studies on alphaviruses in Uruguay". [en línea] American Journal of Tropical Medicine and Hygiene, 2018, 98 (6), 1811-1818. doi: 10.4269/ajtmh.17-0980
ISSN: 0002-9637
Geographic coverage: Uruguay
License: Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)
Appears in Collections:Publicaciones académicas y científicas - Facultad de Ciencias

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