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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Oddone, Natalia | es |
dc.contributor.author | Lecot Calandria, Nicole Valerie | es |
dc.contributor.author | Fernández Lomonaco, Marcelo Luis | es |
dc.contributor.author | Rodríguez-Haralambides, A. | es |
dc.contributor.author | Cabral González, Pablo | es |
dc.contributor.author | Cerecetto, Hugo | es |
dc.contributor.author | Benech, Juan Claudio | es |
dc.date.accessioned | 2019-10-02T22:14:45Z | - |
dc.date.available | 2019-10-02T22:14:45Z | - |
dc.date.issued | 2016 | es |
dc.date.submitted | 20191001 | es |
dc.identifier.citation | Oddone, N., et al. In vitro and in vivo uptake studies of PAMAM G4.5 dendrimers in breast cancer. Journal of Nanobiotechnology, 2016, 14 (1), art. no. 45. doi: 10.1186/s12951-016-0197-6 | es |
dc.identifier.issn | 1477-3155 | es |
dc.identifier.uri | https://hdl.handle.net/20.500.12008/22077 | - |
dc.description.abstract | Background: Breast cancer is the second leading cause of cancer death worldwide. Nanotechnology approaches can overcome the side effects of chemotherapy as well as improve the efficacy of drugs. Dendrimers are nanometric size polymers which are suitable as drug delivery systems. To the best of our knowledge, studies on the application of PAMAM G4.5 (polyamidoamine half generation 4) dendrimers as potential drug delivery systems in breast cancer have not been reported. In this work we developed a PAMAM G4.5 dendrimer containing FITC (fluorescein isothiocyanate) dye to study their uptake by murine breast cancer cells and BALB/c mice breast tumors. Results: We performed a reaction between FITC and PAMAM G4.5 dendrimers which were previously derivatized with piperazine (linker molecule), characterized them by 1H NMR (proton nuclear magnetic resonance) spectroscopy and MALDI-TOF (matrix-assisted laser desorption/ionization- time-of-flight) mass spectrometry. The experimental data indicated that 2 FITC molecules could be bound covalently at the PAMAM G4.5 dendrimer surface, with 17 FITC molecules probably occluded in PAMAM dendrimers cavity. PAMAM-FITC dendrimer (PAMAM G4.5-piperazinyl-FITC dendrimer) size distribution was evaluated by DLS (dynamic light scattering) and TEM (transmission electron microscopy). The nanoparticle hydrodynamic size was 96.3 ± 1.4 nm with a PdI (polydispersion index) of 0.0296 ± 0.0171, and the size distribution measured by TEM was 44.2 ± 9.2 nm. PAMAM-FITC dendrimers were neither cytotoxic in 4T1 cells nor hemolytic up to 24 h of incubation. In addition, they were uptaken in vitro by 4T1 cells and in vivo by BALB/c mice breast tumors. PAMAM G4.5-piperazinyl-FITC dendrimer intracellular distribution was observed through histologic analysis of the tumor by laser confocal microscopy. Conclusion: These results indicate that PAMAM G4.5 dendrimers enter tumor tissue cells, being good candidates to be used as antitumor drug delivery systems for breast cancer treatment and diagnosis. | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | en | es |
dc.publisher | BioMed Central Ltd. | es |
dc.relation.ispartof | Journal of Nanobiotechnology, 2016, 14 (1), art. no. 45 | es |
dc.rights | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad De La República. (Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
dc.subject | Antitumor drug delivery systems | es |
dc.subject | Breast cancer treatment or diagnosis | es |
dc.subject | PAMAM G4.5 dendrimers | es |
dc.title | In vitro and in vivo uptake studies of PAMAM G4.5 dendrimers in breast cancer | es |
dc.type | Artículo | es |
dc.contributor.filiacion | Oddone, Natalia. IIBCE | es |
dc.contributor.filiacion | Lecot Calandria, Nicole Valerie. Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Investigaciones Nucleares | es |
dc.contributor.filiacion | Fernández Lomonaco, Marcelo Luis. Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Investigaciones Nucleares | es |
dc.contributor.filiacion | Cabral González, Pablo. Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Investigaciones Nucleares | es |
dc.contributor.filiacion | Cerecetto, Hugo. Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Investigaciones Nucleares | es |
dc.contributor.filiacion | Benech, Juan Claudio. IIBCE | es |
dc.rights.licence | Licencia Creative Commons Atribución (CC –BY 4.0) | es |
dc.identifier.doi | 10.1186/s12951-016-0197-6 | es |
Aparece en las colecciones: | Publicaciones académicas y científicas - Facultad de Ciencias |
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