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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Tosar Rovira, Juan Pablo | es |
dc.contributor.author | Gámbaro, Fabiana | es |
dc.contributor.author | Darré, Leonardo | es |
dc.contributor.author | Pantano, Sergio | es |
dc.contributor.author | Westhof, E. | es |
dc.contributor.author | Cayota, Alfonso | es |
dc.date.accessioned | 2019-10-02T22:12:07Z | - |
dc.date.available | 2019-10-02T22:12:07Z | - |
dc.date.issued | 2018 | es |
dc.date.submitted | 20190930 | es |
dc.identifier.citation | Tosar., J.P., et al. Dimerization confers increased stability to nucleases in 5 halves from glycine and glutamic acid tRNAs. Nucleic Acids Research, 2018, 46 (17): 9081-9093. doi: 10.1093/nar/gky495 | es |
dc.identifier.issn | 0305-1048 | es |
dc.identifier.uri | https://hdl.handle.net/20.500.12008/22059 | - |
dc.description.abstract | We have previously shown that 5 halves from tRNA Gly GCC and tRNA Glu CUC are the most enriched small RNAs in the extracellular space of human cell lines, and especially in the non-vesicular fraction. Extracellular RNAs are believed to require protection by either encapsulation in vesicles or ribonucleoprotein complex formation. However, deproteinization of non-vesicular tRNA halves does not affect their retention in size-exclusion chromatography. Thus, we considered alternative explanations for their extracellular stability. In-silico analysis of the sequence of these tRNA-derived fragments showed that tRNA Gly 5 halves can form homodimers or heterodimers with tRNA Glu 5 halves. This capacity is virtually unique to glycine tRNAs. By analyzing synthetic oligonucleotides by size exclusion chromatography, we provide evidence that dimerization is possible in vitro. tRNA halves with single point substitutions preventing dimerization are degraded faster both in controlled nuclease digestion assays and after transfection in cells, showing that dimerization can stabilize tRNA halves against the action of cellular nucleases. Finally, we give evidence supporting dimerization of endogenous tRNA Gly GCC 5 halves inside cells. Considering recent reports have shown that 5 tRNA halves from Ala and Cys can form tetramers, our results highlight RNA intermolecular structures as a new layer of complexity in the biology of tRNA-derived fragments. | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | en | es |
dc.publisher | Oxford University Press | es |
dc.relation.ispartof | Nucleic Acids Research, 2018, 46 (17): 9081-9093 | es |
dc.rights | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad De La República. (Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
dc.subject | Amino Acyl-tRNA Synthetases | es |
dc.subject | RNA, Transfer | es |
dc.subject | Genetic code | es |
dc.title | Dimerization confers increased stability to nucleases in 5 halves from glycine and glutamic acid tRNAs | es |
dc.type | Artículo | es |
dc.contributor.filiacion | Tosar Rovira, Juan Pablo. Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Investigaciones Nucleares | es |
dc.contributor.filiacion | Gámbaro, Fabiana. Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares | es |
dc.contributor.filiacion | Darré, Leonardo. Instituto Pasteur (Montevideo) | es |
dc.contributor.filiacion | Pantano, Sergio. Instituto Pasteur (Montevideo) | es |
dc.contributor.filiacion | Cayota, Alfonso. Instituto Pasteur (Montevideo) | es |
dc.rights.licence | Licencia Creative Commons Atribución – No Comercial (CC-BY-NC- 4.0) | es |
dc.identifier.doi | 10.1093/nar/gky495 | es |
Aparece en las colecciones: | Publicaciones académicas y científicas - Facultad de Ciencias |
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101093nargky495.pdf | 5,75 MB | Adobe PDF | Visualizar/Abrir |
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