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T908 polymeric micelles improved the uptake of Sgc8-c aptamer probe in tumor-bearing mice: a co-association study between the probe and preformed nanostructures
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Autor:
Castelli, Romina
Ibarra, Manuel
Faccio, Ricardo
Miraballes, Iris
Fernández, Marcelo
Moglioni, Albertina
Cabral, Pablo
Cerecetto, Hugo
Glisoni, Romina J.
Calzada, Victoria
Ibarra, Manuel
Faccio, Ricardo
Miraballes, Iris
Fernández, Marcelo
Moglioni, Albertina
Cabral, Pablo
Cerecetto, Hugo
Glisoni, Romina J.
Calzada, Victoria
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MICELAS POLIMERICAS
APTAMERO SGC8-C
ORIENTACION ACTIVA
SONDAS
TUMOR
APTAMERO SGC8-C
ORIENTACION ACTIVA
SONDAS
TUMOR
Año de publicación:
2022
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Resumen:
Aptamers are oligonucleotides that have the characteristic of recognizing a target with high affinity and specificity. Based on our previous studies, the aptamer probe Sgc8-c-Alexa647 is a promising tool for molecular imaging of PTK7, which is an interesting biomarker in cancer. In order to improve the delivery of this probe as well as create a novel drug delivery nanosystem targeted to the PTK7 receptor, we evaluate the co-association between the probe and preformed nanostructures. In this work, preformed pegylated liposomes (PPL) and linear and branched pristine polymeric micelles (PMs), based on PEO–PPO–PEO triblock copolymers were used: poloxamer F127® and poloxamines T1307® and T908®. For it, Sgc8-c-Alexa647 and its co-association with the different nanostructures was exhaustively analyzed. DLS analysis showed nanometric sizes, and TEM and AFM showed notable differences between free- and co-associated probe. Likewise, all nanosystems were evaluated on A20 lymphoma cell line overexpressing PTK7, and the confocal microscopy images showed distinctness in cellular uptake. Finally, the biodistribution in BALB/c mice bearing lymphoma-tumor and pharmacokinetic study revealed an encouraging profile for T908-probe. All data obtained from this work suggested that PMs and, more specifically T908 ones, are good candidates to improve the pharmacokinetics and the tumor uptake of aptamer-based probes.
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MDPI
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Pharmaceuticals v.15, n°15, 2022. -- 20 h.
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Castelli, R, Ibarra, M, Faccio, R, y otros. "T908 polymeric micelles improved the uptake of Sgc8-c aptamer probe in tumor-bearing mice: a co-association study between the probe and preformed nanostructures". Pharmaceuticals. [en línea] 2022, vol. 15, n°15, 20 h. DOI: https://doi.org/10.3390/ph15010015
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Licencia Creative Commons Atribución (CC - By 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| AA Castelli, Romina. Pharmaceuticals v.15, n°1. --15010015.pdf | — | 6.42 MB | Adobe PDF |