Por favor, use este identificador para citar o enlazar este ítem: https://hdl.handle.net/20.500.12008/55175

Título:

Lab-made 3D printed stoppers as high-throughput cell migration screening tool

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Autor:

Acosta, Silvina
Canclini, Lucía
Galarraga, Carlos
Justet, Cristian
Alem, Diego

Tutor:

Tipo de documento:

Artículo

Editor:

Palabras clave:

3D-printing
EdU proliferation assay
HaCaT cell line
Cell migration
Wound healing assay

Descriptores:

BIOENSAYO
IMPRESIÓN TRIDIMENSIONAL
MOVIMIENTO CELULAR
CÉLULAS HaCaT
ENSAYOS ANALÍTICOS DE ALTO RENDIMIENTO
MÉTODOS
CICATRIZACIÓN DE HERIDAS

Año de publicación:

2022

Contenido:

Resumen:

Cell migration is a process that underlies the development and maintenance of multicellular organisms, with profound implications in various pathologies. The study of cell migration is fundamental in various fields of basic biology and pharmaceutical development. Wound healing assay is an indirect way to assess cell migration. Conventional methods, such as the scratch test, are inexpensive and easy to execute but have the disadvantages of being poorly reproducible and difficult to perform on a high-throughput scale. Meanwhile, commercial strategies are expensive. In the present work, we developed a lab-made wound healing assay device that is inexpensive, easy to handle, and reproducible. We designed 3D-printed stoppers compatible with cell culture in 96-well plates. These stoppers did not affect HaCaT cells viability. The stopper-produced initial wound size was reproducible on a high-throughput scale. Also, stoppers demonstrated their effectiveness to evaluate cell migration and allowed differentiating treatments with and without fetal bovine serum. Finally, proliferation assay was determined in this wound healing model. In conclusion, our lab-made 3D-printed stopper-based assay is a more economical alternative to currently available strategies for developing reproducible, high-throughput assays to assess cell migration and proliferation.

Descripción:

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Editorial:

SAGE Publications

EN:

SLAS Technology. 2022;37(1)-39-43

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Citación:

Acosta S, Canclini L, Galarraga C y otros. Lab-made 3D printed stoppers as high-throughput cell migration screening tool. SLAS Technology [en línea]. 2022;37(1)-39-43

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Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)

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Publicaciones Académicas y Científicas
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Lab made 3D printed stoppers.pdf — 927.82 KB Adobe PDF
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