| Metadato | Etiqueta | Valor | Idioma |
|---|---|---|---|
| dc.contributor.author | Autor | Acosta, Silvina | - |
| dc.contributor.author | Autor | Canclini, Lucía | - |
| dc.contributor.author | Autor | Galarraga, Carlos | - |
| dc.contributor.author | Autor | Justet, Cristian | - |
| dc.contributor.author | Autor | Alem, Diego | - |
| dc.contributor.filiacion | Filiación | Acosta Silvina, Instituto de Investigaciones Biológicas Clemente Estable (Uruguay). Laboratorio de Epigenética e Inestabilidad Genómica | - |
| dc.contributor.filiacion | Filiación | Canclini Lucía, Instituto de Investigaciones Biológicas Clemente Estable (Uruguay). Departamento de Genética | - |
| dc.contributor.filiacion | Filiación | Galarraga Carlos, Universidad ORT (Uruguay). Facultad de Diseño y Comunicación. | - |
| dc.contributor.filiacion | Filiación | Justet Cristian, Universidad de la República (Uruguay). Facultad de Medicina. Departamento de Bioquímica | - |
| dc.contributor.filiacion | Filiación | Alem Diego, Instituto de Investigaciones Biológicas Clemente Estable (Uruguay). Laboratorio de Epigenética e Inestabilidad Genómica | - |
| dc.date.accessioned | Fecha ingreso | 2026-05-25T18:31:55Z | - |
| dc.date.available | Fecha disponible | 2026-05-25T18:31:55Z | - |
| dc.date.issued | Año de publicación | 2022 | - |
| dc.description.abstract | Resumen | Cell migration is a process that underlies the development and maintenance of multicellular organisms, with profound implications in various pathologies. The study of cell migration is fundamental in various fields of basic biology and pharmaceutical development. Wound healing assay is an indirect way to assess cell migration. Conventional methods, such as the scratch test, are inexpensive and easy to execute but have the disadvantages of being poorly reproducible and difficult to perform on a high-throughput scale. Meanwhile, commercial strategies are expensive. In the present work, we developed a lab-made wound healing assay device that is inexpensive, easy to handle, and reproducible. We designed 3D-printed stoppers compatible with cell culture in 96-well plates. These stoppers did not affect HaCaT cells viability. The stopper-produced initial wound size was reproducible on a high-throughput scale. Also, stoppers demonstrated their effectiveness to evaluate cell migration and allowed differentiating treatments with and without fetal bovine serum. Finally, proliferation assay was determined in this wound healing model. In conclusion, our lab-made 3D-printed stopper-based assay is a more economical alternative to currently available strategies for developing reproducible, high-throughput assays to assess cell migration and proliferation. | es |
| dc.format.extent | Extensión | 5 p. | es |
| dc.format.mimetype | Formato | application/pdf | es |
| dc.identifier.citation | Citación | Acosta S, Canclini L, Galarraga C y otros. Lab-made 3D printed stoppers as high-throughput cell migration screening tool. SLAS Technology [en línea]. 2022;37(1)-39-43 | es |
| dc.identifier.doi | DOI | 10.1016/j.slast.2021.10.003 | - |
| dc.identifier.eissn | e-ISSN | 2472-6311 | - |
| dc.identifier.uri | URI | https://hdl.handle.net/20.500.12008/55175 | - |
| dc.language.iso | Idioma | en | es |
| dc.publisher | Editorial | SAGE Publications | es |
| dc.relation.ispartof | EN | SLAS Technology. 2022;37(1)-39-43 | es |
| dc.rights | Derechos | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
| dc.rights.licence | Licencia | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) | es |
| dc.subject | Palabras clave | 3D-printing | es |
| dc.subject | Palabras clave | EdU proliferation assay | es |
| dc.subject | Palabras clave | HaCaT cell line | es |
| dc.subject | Palabras clave | Cell migration | es |
| dc.subject | Palabras clave | Wound healing assay | es |
| dc.subject.other | Descriptores | BIOENSAYO | es |
| dc.subject.other | Descriptores | IMPRESIÓN TRIDIMENSIONAL | es |
| dc.subject.other | Descriptores | MOVIMIENTO CELULAR | es |
| dc.subject.other | Descriptores | CÉLULAS HaCaT | es |
| dc.subject.other | Descriptores | ENSAYOS ANALÍTICOS DE ALTO RENDIMIENTO | es |
| dc.subject.other | Descriptores | MÉTODOS | es |
| dc.subject.other | Descriptores | CICATRIZACIÓN DE HERIDAS | es |
| dc.title | Título | Lab-made 3D printed stoppers as high-throughput cell migration screening tool | es |
| dc.type | Tipo de documento | Artículo | es |
Colecciones:
| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| Lab made 3D printed stoppers.pdf | — | 927.82 KB | Adobe PDF |