Título:
Enhancing pre-sexual and sexual differentiation of Toxoplasma gondii using retinal epithelial cells and intestinal organoids
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Autor:
Cancela Bruno, Saira
Sena, Florencia
Pagotto, Romina
Crispo, Martina
Francia, María Eugenia
Bollati-Fogolín, Mariela
Sena, Florencia
Pagotto, Romina
Crispo, Martina
Francia, María Eugenia
Bollati-Fogolín, Mariela
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Tipo de documento:
Artículo
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Toxoplasma gondii
Life cycle
Stage conversion
Sexual differentiation
in vitro models
Intestinal organoids
Feline intestinal environment
Life cycle
Stage conversion
Sexual differentiation
in vitro models
Intestinal organoids
Feline intestinal environment
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Año de publicación:
2025
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Resumen:
Toxoplasmosis, caused by Toxoplasma gondii, is a major global health concern due to its high prevalence, zoonotic transmission, and economic impact on livestock. The parasite’s life cycle includes asexual, pre-sexual, and sexual stages, the latter responsible for oocyst shedding and genetic recombination. Understanding sexual differentiation is critical, but access has been limited because these stages occur only in the feline intestinal epithelium. Recent studies identify host metabolic cues and the microrchidia (MORC) protein complex as regulators of sexual commitment. We optimize in vitro approaches to enrich pre-sexual and sexual stages by combining a human retinal epithelial cell line and murine intestinal organoids with FELIX medium, which mimics feline intestinal biochemistry, and conditional MORC depletion. This system increases stage-specific gene expression and marker detection, demonstrating synergistic effects of host environment and genetic regulation. Our findings provide accessible models to study T. gondii sexual differentiation, with implications for controlling transmission and genetic diversity.
Descripción:
Artículo publicado en el marco del Doctorado en Biotecnología de Saira Cancela Bruno.
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Editorial:
Cell Press
EN:
Cell Reports, 2025, 44(10): 116451
Financiadores:
ANII: FMV_1_2019_156213
Citación:
Cancela Bruno, S, Sena, F, Pagotto, R, [y otros autores]. "Enhancing pre-sexual and sexual differentiation of Toxoplasma gondii using retinal epithelial cells and intestinal organoids". Cell Reports. [en línea] 2025, 44(10): 116451. 17 h. DOI10.1016/j.celrep.2025.116451
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Licencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| 10.1016.j.celrep.2025.116451.pdf | — | 5.49 MB | Adobe PDF |