| Metadato | Etiqueta | Valor | Idioma |
|---|---|---|---|
| dc.contributor.author | Autor | Cancela Bruno, Saira | - |
| dc.contributor.author | Autor | Sena, Florencia | - |
| dc.contributor.author | Autor | Pagotto, Romina | - |
| dc.contributor.author | Autor | Crispo, Martina | - |
| dc.contributor.author | Autor | Francia, María Eugenia | - |
| dc.contributor.author | Autor | Bollati-Fogolín, Mariela | - |
| dc.contributor.filiacion | Filiación | Cancela Bruno Saira, Instituto Pasteur (Montevideo). | - |
| dc.contributor.filiacion | Filiación | Sena Florencia, Instituto Pasteur (Montevideo). | - |
| dc.contributor.filiacion | Filiación | Pagotto Romina, Instituto Pasteur (Montevideo). | - |
| dc.contributor.filiacion | Filiación | Crispo Martina, Instituto Pasteur (Montevideo). | - |
| dc.contributor.filiacion | Filiación | Francia María Eugenia, Instituto Pasteur (Montevideo). | - |
| dc.contributor.filiacion | Filiación | Bollati-Fogolín Mariela, Instituto Pasteur (Montevideo). | - |
| dc.date.accessioned | Fecha ingreso | 2025-11-05T17:45:50Z | - |
| dc.date.available | Fecha disponible | 2025-11-05T17:45:50Z | - |
| dc.date.issued | Año de publicación | 2025 | - |
| dc.description | Descripción | Artículo publicado en el marco del Doctorado en Biotecnología de Saira Cancela Bruno. | es |
| dc.description.abstract | Resumen | Toxoplasmosis, caused by Toxoplasma gondii, is a major global health concern due to its high prevalence, zoonotic transmission, and economic impact on livestock. The parasite’s life cycle includes asexual, pre-sexual, and sexual stages, the latter responsible for oocyst shedding and genetic recombination. Understanding sexual differentiation is critical, but access has been limited because these stages occur only in the feline intestinal epithelium. Recent studies identify host metabolic cues and the microrchidia (MORC) protein complex as regulators of sexual commitment. We optimize in vitro approaches to enrich pre-sexual and sexual stages by combining a human retinal epithelial cell line and murine intestinal organoids with FELIX medium, which mimics feline intestinal biochemistry, and conditional MORC depletion. This system increases stage-specific gene expression and marker detection, demonstrating synergistic effects of host environment and genetic regulation. Our findings provide accessible models to study T. gondii sexual differentiation, with implications for controlling transmission and genetic diversity. | es |
| dc.description.sponsorship | Financiadores | ANII: FMV_1_2019_156213 | es |
| dc.format.extent | Extensión | 17 h. | es |
| dc.format.mimetype | Formato | application/pdf | es |
| dc.identifier.citation | Citación | Cancela Bruno, S, Sena, F, Pagotto, R, [y otros autores]. "Enhancing pre-sexual and sexual differentiation of Toxoplasma gondii using retinal epithelial cells and intestinal organoids". Cell Reports. [en línea] 2025, 44(10): 116451. 17 h. DOI10.1016/j.celrep.2025.116451 | es |
| dc.identifier.doi | DOI | 10.1016/j.celrep.2025.116451 | - |
| dc.identifier.eissn | e-ISSN | 2211-1247 | - |
| dc.identifier.uri | URI | https://hdl.handle.net/20.500.12008/52349 | - |
| dc.language.iso | Idioma | en | es |
| dc.publisher | Editorial | Cell Press | es |
| dc.relation.ispartof | EN | Cell Reports, 2025, 44(10): 116451 | es |
| dc.rights | Derechos | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
| dc.rights.licence | Licencia | Licencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0) | es |
| dc.subject | Palabras clave | Toxoplasma gondii | es |
| dc.subject | Palabras clave | Life cycle | es |
| dc.subject | Palabras clave | Stage conversion | es |
| dc.subject | Palabras clave | Sexual differentiation | es |
| dc.subject | Palabras clave | in vitro models | es |
| dc.subject | Palabras clave | Intestinal organoids | es |
| dc.subject | Palabras clave | Feline intestinal environment | es |
| dc.title | Título | Enhancing pre-sexual and sexual differentiation of Toxoplasma gondii using retinal epithelial cells and intestinal organoids | es |
| dc.type | Tipo de documento | Artículo | es |
Colecciones:
| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| 10.1016.j.celrep.2025.116451.pdf | — | 5.49 MB | Adobe PDF |