Título:
Photoreceptor calyceal processes accompany the developing outer segment, adopting a stable length despite a dynamic core
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Autor:
Sharkova, Maria
Aparicio Díaz, Hector Gonzalo
Mouzaaber, Constantin
Zolessi, Flavio R.
Hocking, Jennifer C.
Aparicio Díaz, Hector Gonzalo
Mouzaaber, Constantin
Zolessi, Flavio R.
Hocking, Jennifer C.
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Tipo de documento:
Artículo
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Palabras clave:
Photoreceptors
Actin
Microvilli
Zebrafish
Retinal pigment epithelium
Müller glia
Actin
Microvilli
Zebrafish
Retinal pigment epithelium
Müller glia
Descriptores:
Año de publicación:
2024
Contenido:
Resumen:
Vertebrate photoreceptors detect light through a large cilium-based outer segment, which is filled with photopigment-laden membranous discs. Surrounding the base of the outer segment are microvilli-like calyceal
processes (CPs). While CP disruption has been associated with altered outer segment morphology and
photoreceptor degeneration, the role of the processes remains elusive. Here, we used zebrafish as a model to
characterize CPs. We quantified CP parameters and report a strong disparity in outer segment coverage between
photoreceptor subtypes. CP length is stable across light and dark conditions, while heat shock inducible expression of tagged actin revealed rapid turnover of the CP actin core. Detailed imaging of the embryonic
retina uncovered substantial remodeling of the developing photoreceptor apical surface, including a transition
from dynamic tangential processes to vertically-oriented CPs immediately prior to outer segment formation.
Remarkably, we also found a direct connection between apical extensions of the Müller glia and retinal pigment
epithelium, arranged as bundles around the ultraviolet sensitive cones. In summary, our data characterize the
structure, development, and surrounding environment of photoreceptor microvilli in the zebrafish retina.
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Versión aceptada (Postprint)
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Editorial:
The Company of Biologists
EN:
Journal of Cell Science, 2024.
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Citación:
Sharkova, M, Aparicio Díaz, H, Mouzaaber, C [y otros autores]. "Photoreceptor calyceal processes accompany the developing outer segment, adopting a stable length despite a dynamic core". Journal of Cell Science. 2024. 36 h. DOI: 10.1242/jcs.261721.
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ISSN:
1477-9137
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Licencia:
Licencia Creative Commons Atribución (CC - By 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| 10.1242.jcs.261721.pdf | — | 1.76 MB | Adobe PDF |