Título:
SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse
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Autor:
Capoano Bevilacqua, Carlos Adrián
Ortiz-Laquintana, Luis Adrián
Rodríguez-Casuriaga, Rosana
Schlapp, Geraldine
Meikle, María Noel
Mulet, Ana Paula
Crispo, Martina
Benavente, Ricardo
Geisinger Wschebor, Adriana
Ortiz-Laquintana, Luis Adrián
Rodríguez-Casuriaga, Rosana
Schlapp, Geraldine
Meikle, María Noel
Mulet, Ana Paula
Crispo, Martina
Benavente, Ricardo
Geisinger Wschebor, Adriana
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Tipo de documento:
Artículo
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Palabras clave:
SPATS1
Descriptores:
ESPERMATOGENESIS
FERTILIDAD
FERTILIDAD
Año de publicación:
2021
Contenido:
Resumen:
SPATS1 (spermatogenesis-associated, serine-rich 1) is an evolutionarily conserved, testis-specific protein that is differentially expressed during rat male meiotic prophase. Some reports have suggested a link between SPATS1 underexpression/mutation and human pathologies such as male infertility and testicular cancer. Given the absence of functional studies, we generated a Spats1 loss-of-function mouse model using CRISPR/Cas9 technology. The phenotypic analysis showed no overt phenotype in Spats1-/- mice, with both males and females being fertile. Flow cytometry and histological analyses did not show differences in the testicular content and histology between WT and knockout mice. Moreover, no significant differences in sperm concentration, motility, and morphology, were observed between WT and KO mice. These results were obtained both for young adults and for aged animals. Besides, although an involvement of SPATS1 in the Wnt signaling pathway has been suggested, we did not detect changes in the expression levels of typical Wnt pathway-target genes in mutant individuals. Thus, albeit Spats1 alteration might be a risk factor for male testicular health, we hereby show that this gene is not individually essential for male fertility and spermatogenesis in mouse.
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PloS ONE
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PLoS ONE, 2021, 16(5): e0251028
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Citación:
Capoano Bevilacqua, C, Ortiz-Laquintana, L, Rodríguez-Casuriaga, R, [y otros autores]. "SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse". PLoS ONE. [en línea] 2021, 16(5): e0251028. 18 h.
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https://hdl.handle.net/20.500.12008/38147
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ISSN:
1932-6203
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Licencia Creative Commons Atribución (CC - By 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| 101371journalpone0251028.pdf | — | 2.63 MB | Adobe PDF |