https://hdl.handle.net/20.500.12008/33109 Tue, 07 Jul 2026 16:16:12 GMT 2026-07-07T16:16:12Z https://colibri.udelar.edu.uy:443/jspui/retrieve/168208/Logo IH (1).png https://hdl.handle.net/20.500.12008/33109 https://hdl.handle.net/20.500.12008/55924 Título: Coordinated Th1- and Th17-Related Responses Support Antibody- and Neutrophil-Mediated Protection Against Pneumococcal Pneumonia Autor: Rial, Analía; Céspedes, María Paula; Comas, Victoria; Rivera-Patrón, Mariana; Marqués, Juan Martín; Chabalgoity, José Alejandro Resumen: Streptococcus pneumoniae is a leading cause of community-acquired pneumonia, yet the immune mechanisms required for protection against invasive pulmonary infection remain inadequately understood. Using a murine model of homologous protection against invasive pneumococcal pneumonia, we explored the relative contributions of humoral and cellular immunity using adoptive serum transfer, immune cell depletion, and lung transcriptional profiling. Our findings indicated that passive transfer of immune serum provided robust protection, while neutrophil depletion significantly compromised bacterial control, highlighting that both antibodies and neutrophils are key mediators of protection. In contrast, depletion of CD4+ T cells or NK cells did not compromise survival. Although IL-17A has been widely implicated in host defense against pneumococcal infection, IL 17A-deficient mice remained protected, albeit with delayed clearance and reduced early antibody responses. We associate this delay with compensatory upregulation of IL-17F and increased expression of Th1-associated genes in the lungs. Together, these findings indicate that IL-17A is not essential for protection and support a model in which coordinated Th1 and Th17-related cytokine responses collectively promote neutrophil recruitment and effective antibody-mediated defense. These results highlight functional redundancy within the IL-17 cytokine axis and suggest that integrated cytokine networks, rather than individual mediators, underpin protective immunity to pneumococcal pneumonia, with implications for next-generation vaccine design. Thu, 01 Jan 2026 00:00:00 GMT https://hdl.handle.net/20.500.12008/55924 2026-01-01T00:00:00Z https://hdl.handle.net/20.500.12008/55907 Título: Genomics helps to decipher the resistance mechanisms present in a Pseudomonas chlororaphis strain recovered in an HIV patient Autor: Montaña, S.; Lazzaro, T.; Uong, S.; Place, K.; Iriarte, A.; Ocampo, C. V.; Vay, C.; Ramírez, M. S. Descripción: Carta al Editor. Mon, 01 Jan 2018 00:00:00 GMT https://hdl.handle.net/20.500.12008/55907 2018-01-01T00:00:00Z https://hdl.handle.net/20.500.12008/55906 Título: Comparison of transcriptomic landscapes of different lamb muscles using RNA-Seq Autor: Armstrong, Eileen; Iriarte, Andres; Nicolini, Paula; De Los Santos, Jorge; Ithurralde, Javier; Bielli, Alejandro; Bianchi, Gianni; Peñagaricano, Francisco Resumen: Transcriptome deep sequencing is a powerful tool for exploring the genetic architecture of complex traits. Gene expression patterns may explain a high degree of the observed pheno typic differences in histochemical and metabolic parameters related to meat quality among different muscles. In this study, we sequenced by RNA-Seq the whole transcriptome of nine lamb muscles: Semimembranosus(SM), Semitendinosus (ST), Cranial gluteobiceps, Gluteus medius (GM), Rectus femoris, Supraspinatus (SS), Longissimus lumborum (LL), Adductor and Psoas major. Significant gene expression differences were detected between almost all pairwise comparisons, being more pronounced between SS and ST, SM and LL, and ST and GM. These differences can be explained in terms of ATPase and glycolytic activities, muscle fiber typing and oxidative score, clustering muscles as fast glycolytic, intermediate or slow oxidative. ST showed up-regulation of gene pathways related to carbohy drate metabolism, energy generation and protein turnover as expected from a fast white muscle. SS showed myosin isoforms typical of slow muscles and high expression of genes related to calcium homeostasis and vascularization. SM, LL and GM showed in general intermediate gene expression patterns. Several novel transcripts were detected, mostly related to muscle contraction and structure, oxidative metabolism, lipid metabolism and protein phosphorylation. Expression profiles were consistent with previous histochemical and metabolic characterization of these muscles. Up-regulation of ion transport genes may account for significant differences in water holding capacity. High expression of genes related to cell adhesion, cytoskeleton organization, extracellular matrix components and protein phosphorylation may be related to meat yellowness and lower tenderness scores. Differential expression of genes related to glycolytic activity and lactic acid generation among fast, intermediate and slow muscles may explain the detected final meat pH differ ences. These results reveal new candidate genes associated with lamb meat quality, and give a deeper insight into the genetic architecture of these complex traits. Mon, 01 Jan 2018 00:00:00 GMT https://hdl.handle.net/20.500.12008/55906 2018-01-01T00:00:00Z https://hdl.handle.net/20.500.12008/55881 Título: Molecular characterization of KPC 2–positive Klebsiella pneumoniae isolates from a neurosurgical centre in Argentina Autor: Montaña, S.; Hernandez, M.; Fernandez, J. S.; Pennini, M.; Centrón, D.; Sucari, A.; Iriarte, A.; Ramírez, M. S. Resumen: Carbapenem-resistant Enterobacteriaceae is a growing concern worldwide. Klebsiella pneumoniae is an important nosocomial pathogen with a high capacity for nosocomial spread. We described the occurrence of plasmid-encoded KPC-2–harbouring K. pneumoniae isolates recovered from a neurosurgical centre in Argentina. The blaKPC-2 gene was surrounded by ISkpn6 and ISkpn7. Mon, 01 Jan 2018 00:00:00 GMT https://hdl.handle.net/20.500.12008/55881 2018-01-01T00:00:00Z