https://hdl.handle.net/20.500.12008/32 Reúne artículos científicos, ponencias en eventos, reportes técnicos, comunicaciones y otros. 2026-08-22T01:40:09Z https://hdl.handle.net/20.500.12008/56443 Título: Dorsal and median raphe neuronal firing dynamics characterized by nonlinear measures Autor: Pascovich, Claudia; Serantes, Diego; Rodríguez, Alejo; Mateos, Diego; González, Joaquín; Gallo, Diego; Rivas, Mayda; Devera, Andrea; Lagos, Patricia; Rubido, Nicolás; Torterolo, Pablo Resumen: The dorsal (DRN) and median (MRN) raphe are important nuclei involved in similar functions, including mood and sleep, but playing distinct roles. These nuclei have a different composition of neuronal types and set of neuronal connections, which among other factors, determine their neuronal dynamics. Most works characterize the neuronal dynamics using classic measures, such as using the average spiking frequency (FR), the coefficient of variation (CV), and action potential duration (APD). In the current study, to refine the characterization of neuronal firing profiles, we examined the neurons within the raphe nuclei. Through the utilization of nonlinear measures, our objective was to discern the redundancy and complementarity of these measures, particularly in comparison with classic methods. To do this, we analyzed the neuronal basal firing profile in both nuclei of urethane-anesthetized rats using the Shannon entropy (Bins Entropy) of the inter-spike intervals, permutation entropy of ordinal patterns (OP Entropy), and Permutation Lempel-Ziv Complexity (PLZC). Firstly, we found that classic (i.e., FR, CV, and APD) and nonlinear measures fail to distinguish between the dynamics of DRN and MRN neurons, except for the OP Entropy. We also found strong relationships between measures, including the CV with FR, CV with Bins entropy, and FR with PLZC, which imply redundant information. However, APD and OP Entropy have either a weak or no relationship with the rest of the measures tested, suggesting that they provide complementary information to the characterization of the neuronal firing profiles. Secondly, we studied how these measures are affected by the oscillatory properties of the firing patterns, including rhythmicity, bursting patterns, and clock-like behavior. We found that all measures are sensitive to rhythmicity, except for the OP Entropy. Overall, our work highlights OP Entropy as a powerful and useful quantity for the characterization of neuronal discharge patterns. 2024-01-01T00:00:00Z https://hdl.handle.net/20.500.12008/56441 Título: Tomato pomace-derived nitrated fatty acids: Synthesis and antiplatelet activity Autor: Rodríguez, Lyanne; Lagos, Felipe; Mastrogiovanni, Mauricio; Flores, Ana; Plaza, Andrea; Telleria, Francisca; Palomo, Iván; Fuentes, Eduardo; Trostchansky, Andrés Resumen: This study investigates the antiplatelet properties of tomato pulp to combat cardiovascular diseases. Notably, it examines the formation of nitrated fatty acids (NO2-FA) in tomato pomace, renowned for its potential antiplatelet effects. Through diverse assays, including tandem mass spectrometry, microplate-based platelet aggregation, and flow cytometry, the research identifies NO2-OA, NO2-LA, and NO2-LnA as pivotal antiplatelet compounds. It demonstrates the concentration-dependent antiplatelet effects of nitrated tomato pomace against thrombin receptor activator peptide 6 (TRAP-6) and collagen-induced platelet activation, alongside the modulation of platelet activation markers. Additionally, synergistic effects were observed with nitrated tomato pomace extracts. The findings suggest therapeutic potential for NO2-FA derived from tomato pomace in preventing blood clot formation, with nitrated extracts exhibiting superior efficacy compared to non-nitrated ones. This research highlights the promising role of natural products, such as tomato pomace, in mitigating cardiovascular risks and proposes novel strategies for population health enhancement and cardiovascular disease management. 2024-01-01T00:00:00Z https://hdl.handle.net/20.500.12008/56440 Título: Cervicalgia e inserción anómala del músculo elevador de la escápula. Reporte de caso Autor: Schiappacasse-Faundes, Giancarlo; Gómez-Solís, Manuel; Cocio-Arcos, Rolando; Molina- Vásquez, Nicolás; Sáez-Checura, Felipe Resumen: Las variantes anatómicas en la musculatura de la columna vertebral cervical y cintura escapular son relativamente infrecuentes y tienen el potencial de causar anomalías funcionales y posturales, que a su vez podrían provocar dolor crónico y sensación de masa. Se presenta el caso de una mujer de 66 años, en la que por medio de tomografía computarizada se evidencia la inserción anómala de un vientre accesorio del músculo elevador de la escápula, que se inserta en la fascia del músculo serrato anterior y segunda costilla, determinando cervicalgia y asimetría palpable en las partes blandas del cuello. Es importante reportar estas variantes anatómicas con el fin de explicar ciertos trastornos físicos y cuadros clínicos de la región cérvico-escapular, orientando el tratamiento, que en su gran mayoría tienen manejo kinesiológico.; Anatomical variants in the cervical spine musculature and shoulder girdle are relatively uncommon and have the potential to cause functional and postural abnormalities, which in turn could cause chronic pain and a sensation of mass. The case of a 66-year-old woman is presented, in whom a computed tomography scan shows the anomalous insertion of an accessory belly of the levator scapulae muscle, which is inserted into the serratus anterior muscle fascia and second rib, causing cervicalgia and palpable asymmetry in the soft tissues of the neck. It is important to report these anatomical variants to explain certain physical disorders and clinical symptoms of the cervico-scapular region, guiding the treatment, the vast majority of which have kinesiological management. 2024-01-01T00:00:00Z https://hdl.handle.net/20.500.12008/56439 Título: Uterine transposition in a patient with vulvar cancer Autor: Laufer, Joel; Scasso, Santiago; Kimelman, Dana; Olmedo, Clara; Bentancor, Valeria; Soust, Anne; Sotero, Gonzalo; Ribeiro, Reitan Resumen: Objective: To report the first uterine transposition for fertility preservation in a patient with vulvar cancer.Case: A 26-year-old nulliparous patient with stage IIIB vulvar cancer, which was resected with adequate margins and bilateral inguinofemoral lymphadenectomy.Laparoscopic transposition of the uterus to the upper abdomen, outside of the scope of radiation was performed to preserve fertility and ovarian function. After the end of radiotherapy, the uterus was repositioned into the pelvis.Main Outcome Measure: Uterine and ovarian function preservation. Result: The patient recovered her menstrual cycles spontaneously 1 month after the reimplantation and exhibited normal variation in ovarian hormones.Twelve months after the surgery, the uterus was normal and there was no sign of recurrent disease. Conclusion: Uterine transposition might represent a valid option for fertility preservation in women who require pelvic radiotherapy. However, studies that assess its viability, effectiveness, and safety are required. 2024-01-01T00:00:00Z