https://hdl.handle.net/20.500.12008/21981 Incluye artículos, objetos de conferencias, seminarios y jornadas, reportes técnicos, comunicaciones y otros. 2026-04-23T13:33:02Z https://hdl.handle.net/20.500.12008/54553 Título: Digitizing collections to unlock the full potential of palynology: a case study with the Smithsonian palynology collection Autor: Jaramillo, Carlos; Punyasena, Surangi W.; de Alba, Daurys; Arcila, Angelica Resumen: Large palynological collections have been built over decades and contain vital information. However, they are often difficult to access and use effectively. What is the point of having such collections if they are not fully utilizable? To solve this problem,we digitized the Smithsonian palynological collection using both light and confocal microscopy. We digitized the pollen of 12,000 species and took 40 million photos. Our image library will support a wide array of applications, including environmental monitoring, public health, biodiversity studies, paleoclimate, and the analysis of land-scape changes across spatial and temporal scales. It will also aid in geological correlations used in water exploration and in hydrocarbon storage/production.Summary: Palynology is a century-old practice, contributing data to various fields, from geology to medicine and forensics. Palynological analyses are highly time-consuming and involve visually finding, identifying, and counting thousands of palynomorph grains on microscope slides. These analyses are especially challenging in high-diversity tropical settings. Fortunately, the development of deep learning and the capability to digitize entire microscope slides are allowing palynology to enter a new era. Foundational to this transformation is building solid digital collections that can be achieved by digitizing botanical collections. We are digitizing the Smithsonian palynological collections, which contain 18,000 species, most of which are Neotropical taxa in the Graham Pollen Collection. This digital product consists of high-resolution images of different types transmitted light, differential interference contrast, and optical superresolution (Airyscan) which will be freely available and lay the groundwork for training deep-learning models and applying novel image analysis to palynomorph morphology. Image quality matters, so we outline the best practices we have developed throughout the years of imaging and experimentation. High-resolution imaging of palynological collections holds the key to unraveling the full potential that the study of pollen and spores can offer. Descripción: En este artículo participan más de 30 autores. 2025-01-01T00:00:00Z https://hdl.handle.net/20.500.12008/54552 Título: Towards a more integrative approach for environmental decision-making in Brazilian transitional waters: improving biomonitoring surveys with a benthic foraminiferal biotic index Autor: Bouchet, Vincent M. P.; de Mello e Sousa, Silvia Helena; Bonetti, Carla; Burone, Leticia; Belart, Pierre; Duleba, Wania; Francescangeli, Fabio; Frontalini, Fabrizio; Laut, Lazaro; Raposo, Débora S.; Rodrigues, André R.; Trevisan Disaró, Sibelle; Pupo, Daniel Vicente; Damasceno, Fabrício Leandro; Pavard, Jean-Charles; Alves Martins, Maria Virgínia Resumen: This study represents the first attempt to determine the indicator values of benthic foraminiferal species in Brazilian transitional waters. It also uses a regionally adapted species list to explore the potential application of Foram-AMBI, a biotic index for ecological quality. To test this, we assigned 95 living (rose-bengal-stained) benthic foraminiferal species into five ecological groups (EGs), based on the weighted-averaging (WA) optimum and tolerance to the total organic carbon (TOC) contents. Selected and published regional studies were used as the database, while independent Brazilian datasets from Sepetiba Bay and Guanabara Bay – the most polluted regions – were used to validate the ecological group assignments through Foram-AMBI. Furthermore, ecological quality status (EcoQS) criteria adapted to Brazil were developed for Foram-AMBI. The index accurately reflects the degraded environmental conditions in these two ecosystems, with moderate to poor ecological quality status in the most polluted areas. This was further confirmed by significant correlations between Foram-AMBI and TOC in both bays. This study highlights the importance of developing regional species lists and EcoQS criteria for Foram-AMBI, as the accuracy of the Brazilian list was better than that of the European list. While further research across broader pollution gradients is needed, our findings confirm the suitability and reliability of benthic foraminifera as biological indicators for assessing environmental quality in transitional waters. 2025-01-01T00:00:00Z https://hdl.handle.net/20.500.12008/54551 Título: Diet formulated with rice bran fermented by Rhizopus oryzae and Saccharomyces cerevisiae: impacts on zootechnical performance and intestinal gene expression in Zebrafish (Danio rerio) Autor: Lenz, Gabriela; Macedo Martins, Rejane; Riet, Jade; dos Santos Azevedo, Raíza; Cardoso, Arthur; da Silva Nornberg, Bruna Félix; Bessonart, Martín; Magnone Aleman, Larisa; Fernandes Marins, Luis Fernando; Abou Anni, Iuri Salim; Penteado Gonçalves, Tatiane; Christ-Ribeiro, Anelise; Borges Tesser, Marcelo Resumen: The growing demand for aquaculture has driven the search for sustainable practices and utilization of agro-industrial residues. Brown rice bran, an abundant and low-cost by- product, has emerged as a promising raw material. This dissertation aimed to evaluate solid-state fermentation (SSF) of rice bran using the fungus Rhizopus oryzae and the yeast Saccharomyces cerevisiae with the goal of improving its nutritional value for use in diets formulated for zebrafish (Danio rerio). Proximate composition analyses revealed the strong biotransformation potential of Rhizopus oryzae. Fermentation with this fungus resulted in a significant 36.45% increase in protein content, a 51.62% increase in total polyphenols, and a 13.7% reduction in lipid content. In an in vivo experiment, zebrafish fed a diet containing rice bran fermented by R. oryzae showed the best zootechnical performance, with higher weight gain, specific growth rate, and improved feed conversion. Gene expression analysis showed a significant difference only for glut6, which is related to glucose transport. In summary, the fermentation of brown rice bran with Rhizopus oryzae represents an effective strategy to enhance its nutritional profile, establishing it as a viable alternative for the formulation of more sustainable and efficient diets in aquaculture. 2025-01-01T00:00:00Z https://hdl.handle.net/20.500.12008/54550 Título: Inventario de cesio-137 y plomo-210 en suelos de referencia del centro-oeste de Uruguay: base para estudios de erosión y vigilancia radiológica Autor: Tassano, Marcos; Cabral González, Pablo; Cabrera Azpiroz, Mirel Resumen: Este trabajo caracteriza los niveles de inventario, concentración y distribución vertical de ¹³⁷Cs y ²¹⁰Pbₑₓ en suelos de referencia en Uruguay para establecer una línea base que soporte futuras investigaciones sobre erosión y vigilancia radiológica. Se estudiaron siete sitios distribuidos desde el sur (Colonia) hasta el norte (Rivera), cubriendo un gradiente climático representativo. Los inventarios de ¹³⁷Cs resultaron homogéneos espacialmente (357,8 a 365,4 Bq.m⁻²), mostrando una alta concordancia con registros históricos de deposición atmosférica en Buenos Aires, lo que valida estos datos como referencia regional. Por el contrario, los inventarios de ²¹⁰Pbₑₓ (3973 a 8428 Bq.m⁻² en los primeros 15 cm) presentaron una fuerte correlación positiva con la precipitación media anual (R² = 0,92, p = 0,01). Se concluye que los inventarios de ¹³⁷Cs pueden considerarse estables y adecuados como referencia general para estudios de erosión, mientras que para ²¹⁰Pbₑₓ, debido a su sensibilidad ambiental, es necesario determinar referencias locales específicas. Estos resultados aportan una línea base fundamental para evaluar cambios futuros en la contaminación radiológica regional derivada de eventos nucleares potenciales.; This study characterizes inventory levels, concentration, and vertical distribution of ¹³⁷Cs and ²¹⁰Pbₑₓ in reference soils across Uruguay, establishing a baseline for future erosion and radiological monitoring studies. Seven sites were analyzed along a climatic gradient from south (Colonia) to north (Rivera). The ¹³⁷Cs inventories were spatially homogeneous (357.8 – 365.4 Bq.m⁻²) and closely matched historical atmospheric deposition records from Buenos Aires, validating these as regional references. Conversely, ²¹⁰Pbₑₓ inventories (3973 – 8428 Bq.m⁻² in the upper 15 cm) strongly correlated with mean annual precipitation (R² = 0.92, p = 0.01). Results indicate ¹³⁷Cs inventories are stable and suitable as a general reference for erosion studies, whereas local-specific references are necessary for ²¹⁰Pbₑₓ due to its environmental sensitivity. These findings provide a crucial baseline for assessing future radiological contamination from potential nuclear events in the region. 2025-01-01T00:00:00Z