Reúne artículos científicos, ponencias en eventos, reportes técnicos, comunicaciones y otros. https://hdl.handle.net/20.500.12008/32 2026-04-23T10:49:18Z 2026-04-23T10:49:18Z Barros-Poblete, Marisol Bernardes Neto, Saint-Clair Benavides-Cordoba, Vicente Vieira, Rodolfo p. Baz, Manuel Martí, Joan-Daniel Spruit, Martijn A. Torres-Castro, Rodrigo https://hdl.handle.net/20.500.12008/54538 2026-04-22T16:28:13Z 2022-01-01T00:00:00Z

Título: Early mobilization in intensive care unit in Latin America: A survey based on clinical practice Autor: Barros-Poblete, Marisol; Bernardes Neto, Saint-Clair; Benavides-Cordoba, Vicente; Vieira, Rodolfo p.; Baz, Manuel; Martí, Joan-Daniel; Spruit, Martijn A.; Torres-Castro, Rodrigo Resumen: Background: The application of early mobilization (EM) in intensive care units (ICUs) has shown to improve the physical and ventilatory status of critically ill patients, even after ICU stay. This study aimed to describe the practices regarding EM in ICUs in Latin America. Methods: We conducted an observational, cross-sectional study of professionals from all countries in Latin America. Over 3 months, professionals working in ICU units in Latin America were invited to answer the survey, which was designed by an expert committee and incorporated preliminary questions based on studies about EM recommendations. Results: As many as 174 health professionals from 17 countries completed the survey. The interventions carried out within each ICU were active mobilization (90.5%), passive mobilization (85.0%), manual and instrumental techniques for drainage of mucus secretion (81.8%), and positioning techniques (81%). The professionals who most participated in the rehabilitation process in ICUs were physiotherapists (98.7%), intensive care physicians (61.6%), nurses (56.1%), and respiratory therapists (43.8%). In only 36.1% of the ICUs, protocols were established to determine when a patient should begin EM. In 38.1% of the cases, the onset of EM was established by individual evaluation, and in 25.0% of the cases, it was the medical indication to start rehabilitation and EM. Conclusion: This report shows us that EM of critically ill patients is an established practice in our ICUs like in other developed countries.

2022-01-01T00:00:00Z Goubert, Clement Craig, Rory J. Bilat, Agustín F. Peona, Valentina Vogan, Aron A. Protasio, Anna V. https://hdl.handle.net/20.500.12008/54532 2026-04-22T13:00:44Z 2022-01-01T00:00:00Z

Título: A beginner's guide to manual curation of transposable elements Autor: Goubert, Clement; Craig, Rory J.; Bilat, Agustín F.; Peona, Valentina; Vogan, Aron A.; Protasio, Anna V. Resumen: Background: In the study of transposable elements (TEs), the generation of a high confidence set of consensus sequences that represent the diversity of TEs found in a given genome is a key step in the path to investigate these fascinating genomic elements. Many algorithms and pipelines are available to automatically identify putative TE families present in a genome. Despite the availability of these valuable resources, producing a library of high-quality full-length TE consensus sequences largely remains a process of manual curation. This know-how is often passed on from mentor-to-mentee within research groups, making it difficult for those outside the field to access this highly specialised skill. Results: Our manuscript attempts to fill this gap by providing a set of detailed computer protocols, software recommendations and video tutorials for those aiming to manually curate TEs. Detailed step-by-step protocols, aimed at the complete beginner, are presented in the Supplementary Methods. Conclusions: The proposed set of programs and tools presented here will make the process of manual curation achievable and amenable to all researchers and in special to those new to the field of TEs.

2022-01-01T00:00:00Z Acevedo Tobler, Daniel Hermosilla, Santiago Otero, Natalia Kenny, Juan Del Castillo, Juan https://hdl.handle.net/20.500.12008/54516 2026-04-21T16:01:30Z 2022-01-01T00:00:00Z

Título: Anterior cruciate ligament reconstruction, can an anatomic femoral tunnel be achieved with the trans-tibial technique? Cadaveric study Autor: Acevedo Tobler, Daniel; Hermosilla, Santiago; Otero, Natalia; Kenny, Juan; Del Castillo, Juan Resumen: Purpose: To evaluate the possibility to access the anatomic femoral insertion of the anterior cruciate ligament (ACL) through trans-tibial (TT) and trans-portal technique, for ACL reconstruction in an independent way. To register anatomical characteristics of the TT tunnels. Methods: Ten formaldehyde preserved knee anatomic articular specimens were dissected. Femoral tunnels were confectioned reproducing the original topography of the ACL. First, the femoral tunnel was made with the independent trans-portal technique. Then, utilizing the tibial stump of the ACL and tibial guides at 45°, the TT tunnels were confectioned trying to match the previously made femoral tunnel by trans-portal technique. Results: In all specimens, match between the TT tunnel with the independent trans-portal tunnel was achieved. Mean values for TT coronal angle was 53,0°, for transversal angle 43,3° and for distance from tunnel to joint line 2,55 cm. A horizontalization and medialization of the TT tunnels had to be made to adequately match with the femoral tunnel made by the independent trans-portal technique. Conclusions: By macroscopic anatomic and independent means, an anatomic femoral tunnel was confectioned with the TT technique matching with the anatomic femoral tunnel made in an independent way. As clinical relevance, the present study allows to anatomically assess the possibility to perform an anatomic femoral tunnel through the TT technique. Level of evidence: V

2022-01-01T00:00:00Z Sánchez Fernández, Alba Zandee, Stephanie Mastrogiovanni, Mauricio Charabati, Marc Rubbo, Homero Prat, Alexandre López-Vales, Rubèn https://hdl.handle.net/20.500.12008/54515 2026-04-21T16:00:55Z 2022-01-01T00:00:00Z

Título: Administration of Maresin-1 ameliorates the physiopathology of experimental autoimmune encephalomyelitis Autor: Sánchez Fernández, Alba; Zandee, Stephanie; Mastrogiovanni, Mauricio; Charabati, Marc; Rubbo, Homero; Prat, Alexandre; López-Vales, Rubèn Resumen: Background: Resolution of inflammation is an active and regulated process that leads to the clearance of cell debris and immune cells from the challenged tissue, facilitating the recovery of homeostasis. This physiological response is coordinated by endogenous bioactive lipids known as specialized pro-resolving mediators (SPMs). When resolution fails, inflammation becomes uncontrolled leading chronic inflammation and tissue damage, as occurs in multiple sclerosis (MS). Methods: SPMs and the key biosynthetic enzymes involved in SPM production were analysed by metabololipidomics and qPCR in active brain lesions, serum and peripheral blood mononuclear cells (PBMC) of MS patients as well as in the spinal cord of mice with experimental autoimmune encephalomyelitis (EAE). We also tested the therapeutic actions of the SPM coined Maresin-1 (MaR1) in EAE mice and studied its impact on inflammation by doing luminex and flow cytometry analysis. Results: We show that levels of MaR1 and other SPMs were below the limit of detection or not increased in the spinal cord of EAE mice, whereas the production of pro-inflammatory eicosanoids was induced during disease progression. Similarly, we reveal that SPMs were undetected in serum and active brain lesion samples of MS patients, which was linked to impaired expression of the enzymes involved in the biosynthetic pathways of SPMs. We demonstrate that exogenous administration of MaR1 in EAE mice suppressed the protein levels of various pro-inflammatory cytokines and reduced immune cells counts in the spinal cord and blood. MaR1 also decreased the numbers of Th1 cells but increased the accumulation of regulatory T cells and drove macrophage polarization towards an anti-inflammatory phenotype. Importantly, we provide clear evidence that administration of MaR1 in mice with clinical signs of EAE enhanced neurological outcomes and protected from demyelination. Conclusions: This study reveals that there is an imbalance in the production of SPMs in MS patients and in EAE mice, and that increasing the bioavailability of SPMs, such as MaR1, minimizes inflammation and mediates therapeutic actions. Thus, these data suggest that immunoresolvent therapies, such as MaR1, could be a novel avenue for the treatment of MS.

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