Colibri Colección : Reúne artículos de publicaciones periódicas en donde por lo menos uno de sus autores pertenece a la Facultad de Agronomía.Reúne artículos de publicaciones periódicas en donde por lo menos uno de sus autores pertenece a la Facultad de Agronomía.https://hdl.handle.net/20.500.12008/100642024-03-29T15:15:47Z2024-03-29T15:15:47ZHepatic metabolism of grazing cows of two Holstein strains under two feeding strategies with different levels of pasture inclusionGarcía-Roche Saracco, María MercedesTalmón Travers, DanielCañibe, GuillermoAstessiano, Ana LauraCassina, AdrianaQuijano, DanielCarriquiry Fossemale, MarianaMendoza, Alejandrohttps://hdl.handle.net/20.500.12008/430762024-03-14T13:28:14Z2023-01-01T00:00:00ZTítulo: Hepatic metabolism of grazing cows of two Holstein strains under two feeding strategies with different levels of pasture inclusion
Autor: García-Roche Saracco, María Mercedes; Talmón Travers, Daniel; Cañibe, Guillermo; Astessiano, Ana Laura; Cassina, Adriana; Quijano, Daniel; Carriquiry Fossemale, Mariana; Mendoza, Alejandro
Resumen: The objective of the study was to characterize adaptations of hepatic metabolism of dairy
cows of two Holstein strains with varying proportions of grazing in the feeding strategy. Multiparous
autumn calving Holstein cows of New Zealand (NZH) and North American (NAH)
strains were assigned to a randomized complete block design with a 2 x 2 factorial arrangement
with two feeding strategies that varied in the proportions of pasture and supplementation:
maximum pasture and supplementation with a pelleted concentrate (MaxP) or fixed
pasture and supplementation with a total mixed ration (FixP) from May through November
of 2018. Hepatic biopsies were taken at - 45 ± 17, 21 ± 7, 100 ± 23 and 180 ± 23 days in milk
(DIM), representing prepartum, early lactation, early mid-lactation and late mid-lactation.
The effects of DIM, feeding strategy (FS), strain and their interactions were analyzed with
mixed models using repeated measures. Cows of both strains had similar triglyceride levels,
mitochondrial function and carnitine palmitoyltransferase activity in liver during lactation.
However, there was an effect of DIM and FS as liver triglyceride was higher for the MaxP
strategy at 21 DIM and both mitochondrial function and carnitine palmitoyltransferase activity
in liver were lower for the MaxP strategy at 21 DIM. Hepatic mitochondrial function and
acetylation levels were affected by the interaction between strain and feeding strategy as
both variables were higher for NAH cows in the MaxP strategy. Mid-lactation hepatic gene
expression of enzymes related to fatty acid metabolism and nuclear receptors was higher
for NZH than NAH cows. This work confirms the association between liver triglyceride,
decreased hepatic mitochondrial function and greater mitochondrial acetylation levels in
cows with a higher inclusion of pasture and suggests differential adaptative mechanisms
between NAH and NZH cows to strategies with varying proportions of grazing in the feeding
strategy.2023-01-01T00:00:00ZDifferential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based systemGarcía-Roche Saracco, María MercedesTalmón Travers, DanielCañibe, GuillermoAstessiano, Ana LauraMendoza, AlejandroQuijano, DanielCassina, AdrianaCarriquiry Fossemale, Marianahttps://hdl.handle.net/20.500.12008/430742024-03-14T12:30:59Z2022-01-01T00:00:00ZTítulo: Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system
Autor: García-Roche Saracco, María Mercedes; Talmón Travers, Daniel; Cañibe, Guillermo; Astessiano, Ana Laura; Mendoza, Alejandro; Quijano, Daniel; Cassina, Adriana; Carriquiry Fossemale, Mariana
Resumen: The objective of this study was to assess hepatic
ATP synthesis in Holstein cows of North American and
New Zealand origins and the gluconeogenic pathway,
one of the pathways with the highest ATP demands
in the ruminant liver. Autumn-calving Holstein cows
of New Zealand and North American origins were
managed in a pasture-based system with supplementation
of concentrate that represented approximately
33% of the predicted dry matter intake during 2017,
2018, and 2019, and hepatic biopsies were taken during
mid-lactation at 174 ± 23 days in milk. Cows of
both strains produced similar levels of solids-corrected
milk, and no differences in body condition score were
found. Plasma glucose concentrations were higher for
cows of New Zealand versus North American origin.
Hepatic mitochondrial function evaluated measuring
oxygen consumption rates showed that mitochondrial
parameters related to ATP synthesis and maximum
respiratory rate were increased for cows of New Zealand
compared with North American origin. However,
hepatic gene expression of pyruvate carboxylase, phosphoenolpyruvate
carboxykinase, and pyruvate dehydrogenase
kinase was increased in North American compared
with New Zealand cows. These results altogether
suggest an increased activity of the tricarboxylic cycle
in New Zealand cows, leading to increased ATP synthesis,
whereas North American cows pull tricarboxylic
cycle intermediates toward gluconeogenesis. The fact
that this occurs during mid-lactation could account
for the increased persistency of North American cows,
especially in a pasture-based system. In addition, we
observed an augmented mitochondrial density in New
Zealand cows, which could be related to feed efficiency mechanisms. In sum, our results contribute to the elucidation of hepatic molecular mechanisms in dairy cows
in production systems with higher inclusion of pastures.2022-01-01T00:00:00ZHepatic energy reserves and carnitine palmitoytransferase activity in grazing dairy cows of two pasture-based systems during lactationGarcía-Roche Saracco, María MercedesCañibe, GuillermoAstessiano, A.Talmón Travers, DanielMendoza, A.Cassina, A.Quijano, C.Carriquiry Fossemale, Marianahttps://hdl.handle.net/20.500.12008/430592024-03-13T19:03:11Z2022-01-01T00:00:00ZTítulo: Hepatic energy reserves and carnitine palmitoytransferase activity in grazing dairy cows of two pasture-based systems during lactation
Autor: García-Roche Saracco, María Mercedes; Cañibe, Guillermo; Astessiano, A.; Talmón Travers, Daniel; Mendoza, A.; Cassina, A.; Quijano, C.; Carriquiry Fossemale, Mariana
Descripción: Summary presented: Proccedings of the 7th EAAP International Symposium on Energy and Protein Metabolism and Nutrition (ISEP2022), 12th - 15th, September 2022, Granada, Spain.2022-01-01T00:00:00ZDiferential fates for gluconeorgenic precursors in diverging Holstein genotypesGarcía-Roche Saracco, María MercedesCañibe, GuillermoTalmón Travers, DanielMendoza, A.Quijano, C.Cassina, A.Carriquiry Fossemale, Marianahttps://hdl.handle.net/20.500.12008/430572024-03-13T16:42:03Z2020-01-01T00:00:00ZTítulo: Diferential fates for gluconeorgenic precursors in diverging Holstein genotypes
Autor: García-Roche Saracco, María Mercedes; Cañibe, Guillermo; Talmón Travers, Daniel; Mendoza, A.; Quijano, C.; Cassina, A.; Carriquiry Fossemale, Mariana
Descripción: Resumen2020-01-01T00:00:00Z