Título:
Cannabis sativa extracts inhibit LDL oxidation and the formation of foam cells in vitro, acting as potential multi-step inhibitors of atherosclerosis development
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Autor:
Musetti, Bruno
Kun González, Alejandra E.
Menchaca, David
Rodríguez-Haralambides, A.
Varela Ubillos, Javier
Thomson, Leonor
Bahnson, Edward M.
Kun González, Alejandra E.
Menchaca, David
Rodríguez-Haralambides, A.
Varela Ubillos, Javier
Thomson, Leonor
Bahnson, Edward M.
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Tipo de documento:
Artículo
Editor:
Palabras clave:
Cannabinoids
Macrophages
Oxidation
Atherosclerosis
Cannabis sativa
Cannabis
Antioxidants
Fluorescence
Macrophages
Oxidation
Atherosclerosis
Cannabis sativa
Cannabis
Antioxidants
Fluorescence
Descriptores:
Año de publicación:
2024
Contenido:
Resumen:
Atherosclerotic disease is the leading cause of death world-wide. Our goal was to explore the effect of phytocannabinoids on the molecular mechanisms triggering the development of the atheromatous lesion. Three cannabis sativa extracts of different chemotypes were chemically characterized by UPLC-DAD. The capacity of the extracts to prevent the oxidation of LDL, the formation of foam cells and the activation of an inflammatory response by J774 cells, were monitored by UV-Vis spectrometry, confocal-microscopy and western blot. Three
varieties of cannabis sativa, with high (E1), intermediate (E2) and low (E3) THC/CBD ratios were selected. The three cannabis extracts inhibited the oxidation of LDL by copper ions and the formation of foam cells by J774.1 cells challenged with oxLDL (ED50 5–12 μg mL-1). The effect of the cannabinoid extracts on the endocytic process was independent of the canonical cannabinoid receptors, CB1 and CB2, but related to the action of non-canonical receptors (TRPV1, TRPV4 and GPR55), involved in calcium signaling. Decreased levels of CD36 and
OLR1 scavenger receptors were, at least partially, responsible for the diminished uptake of oxLDL induced by phytocannabinoids. The downregulation of CD36 and OLR1 could be explained by the observed inhibitory effect of the cannabis extracts on the activation of the NFκB pathway by oxLDL. Phytocannabinoids interfere with the main events leading to the development of the atheromatous plaque, opening new venues on atherosclerosis therapy.
Descripción:
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Editorial:
PLoS ONE
EN:
PLoS ONE, 2024, 19(12): e0310777.
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Citación:
Musetti, B, Kun González, A, Menchaca, D [y otros autores]. "Cannabis sativa extracts inhibit LDL oxidation and the formation of foam cells in vitro, acting as potential multi-step inhibitors of atherosclerosis development". PLoS ONE. [en línea] 2024, 19(12): e0310777. 26 h. DOI: 10.1371/journal.pone.0310777
Citación:
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ISSN:
1932-6203
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Licencia:
Licencia Creative Commons Atribución (CC - By 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| 10.1371-journal.pone.0310777.pdf | — | 2.3 MB | Adobe PDF |