Título:
Unraveling the molecular basis of membrane-associated release of coxsackievirus B3
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Autor:
Arce Rama, Rodrigo
Soñora, Martín
Andreu-Moreno, Iván
Moreno Karlen, María del Pilar
Moratorio, Gonzalo
Sanjuán, Rafael
Soñora, Martín
Andreu-Moreno, Iván
Moreno Karlen, María del Pilar
Moratorio, Gonzalo
Sanjuán, Rafael
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Tipo de documento:
Artículo
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Palabras clave:
Coxsackievirus B3
Viral release
Viral spread
Plaque size
Enteroviruses
Viral release
Viral spread
Plaque size
Enteroviruses
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Año de publicación:
2025
Contenido:
Resumen:
Coxsackievirus B3 (CVB3), a member of the Enterovirus genus within the Picornaviridae family, has emerged as a key model for studying viral evolution and pathogenesis. Although traditionally considered obligate lytic viruses, recent research reveals that enteroviruses can also be released nonlytically within extracellular vesicles (EVs). This study explores the impact of mutations at position 63 of the VP3 capsid protein on CVB3 fitness and release mechanisms by substituting asparagine at this position with aromatic, charged, and aliphatic amino acids. We show that mutations at position 63 significantly affect viral release mechanisms and viral spread in cell culture. Specifically, aromatic mutations (N63H, N63Y, N63F, N63W) and the N63D mutation reduce the release of membrane-associated viral particles, while aromatic residues increase viral spread in cell culture and plaque size under specific conditions. These findings suggest that N63 mutations alter protomer interactions, influencing viral release, spread, and plaque formation, providing insights into the molecular mechanisms of CVB3 egress.
Descripción:
Información adicional en: https://doi.org/10.1038/s41598-025-92289-x
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Editorial:
Nature
EN:
Scientific reports, 2025, 15: 8314
Financiadores:
ANII: MOV_CA_2021_1_171985
Citación:
Arce Rama, R, Soñora, M, Andreu-Moreno, I [y otros autores]. "Unraveling the molecular basis of membrane-associated release of coxsackievirus B3". Scientific reports. [en línea] 2025, 15: 8314. 10 h. DOI: 10.1038/s41598-025-92289-x
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ISSN:
2045-2322
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Licencia:
Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| 10.1038.s41598-025-92289-x.pdf | — | 4.21 MB | Adobe PDF |