| Metadato | Etiqueta | Valor | Idioma |
|---|---|---|---|
| dc.contributor.author | Autor | Costábile Cristech, Alicia | - |
| dc.contributor.author | Autor | Domínguez, María Fernanda | - |
| dc.contributor.author | Autor | Guarnaschelli, Inés | - |
| dc.contributor.author | Autor | Preza Pérez, Matías Facundo | - |
| dc.contributor.author | Autor | Koziol, Uriel | - |
| dc.contributor.author | Autor | Castillo Presa, Estela Beatriz | - |
| dc.contributor.author | Autor | Tort, José F. | - |
| dc.contributor.filiacion | Filiación | Costábile Cristech Alicia, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. | - |
| dc.contributor.filiacion | Filiación | Domínguez María Fernanda, Universidad de la República (Uruguay). Facultad de Medicina. | - |
| dc.contributor.filiacion | Filiación | Guarnaschelli Inés, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. | - |
| dc.contributor.filiacion | Filiación | Preza Pérez Matías Facundo, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. | - |
| dc.contributor.filiacion | Filiación | Koziol Uriel, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. | - |
| dc.contributor.filiacion | Filiación | Castillo Presa Estela Beatriz, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. | - |
| dc.contributor.filiacion | Filiación | Tort José F., Universidad de la República (Uruguay). Facultad de Medicina. | - |
| dc.date.accessioned | Fecha ingreso | 2025-04-04T13:46:02Z | - |
| dc.date.available | Fecha disponible | 2025-04-04T13:46:02Z | - |
| dc.date.issued | Año de publicación | 2024 | - |
| dc.description | Descripción | Material suplementario en: 10.3389/fpara.2024.1362199/full#supplementary-material | es |
| dc.description.abstract | Resumen | Flatworms depend on stem cells for continued tissue growth and renewal during their life cycles, making these cells valuable drug targets. While neoblasts are extensively characterized in the free-living planarian Schmidtea mediterranea, and similar stem cells have been characterized in the trematode Schistosoma mansoni, their identification and characterization in cestodes is just emerging. Since stem cells are generally affected by irradiation, in this work we used this experimental approach to study the stem cells of the model cestode Mesocestoides corti. We found that gamma irradiation produces a dosedependent decrease in proliferative cells, requiring higher doses than in other flatworms to completely abolish proliferation. The treatment results in the downregulation of candidate marker genes. Transcriptomic studies reveal that several genes downregulated after irradiation are conserved with other flatworms, and are related to cell cycle, DNA replication and repair functions. Furthermore, proliferative cells were isolated by cell sorting and also characterized transcriptomically. We found that the set of genes characteristic of proliferative cells agrees well with those downregulated during irradiation, and have a significant overlap with those expressed in planarian neoblasts or S. mansoni stem cells. Our study highlights that conserved mechanisms of stem cell biology may be functional in flatworms, suggesting that these could be relevant targets to evaluate in the control of parasitic species. | es |
| dc.description.sponsorship | Financiadores | ANII: FCE_2_2011_1_6503 | es |
| dc.format.extent | Extensión | 19 h | es |
| dc.format.mimetype | Formato | application/pdf | es |
| dc.identifier.citation | Citación | Costábile Cristech, A, Domínguez, M, Guarnaschelli, I [y otros autores]. "Purification and transcriptomic characterization of proliferative cells of Mesocestoides corti selectively affected by irradiation". Frontiers in Parasitology. [en línea] 2024, 3: 1362199. 19 h. DOI: 10.3389/fpara.2024.1362199 | es |
| dc.identifier.doi | DOI | 10.3389/fpara.2024.1362199 | - |
| dc.identifier.issn | ISSN | 2813-2424 | - |
| dc.identifier.uri | URI | https://hdl.handle.net/20.500.12008/49592 | - |
| dc.language.iso | Idioma | en | es |
| dc.publisher | Editorial | Frontiers | es |
| dc.relation.ispartof | EN | Frontiers in Parasitology, 2024, 3: 1362199. | es |
| dc.rights | Derechos | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
| dc.rights.licence | Licencia | Licencia Creative Commons Atribución (CC - By 4.0) | es |
| dc.subject | Palabras clave | Cestodes | es |
| dc.subject | Palabras clave | Germinative cells | es |
| dc.subject | Palabras clave | Proliferation | es |
| dc.subject | Palabras clave | Irradiation | es |
| dc.subject | Palabras clave | Cell purification | es |
| dc.subject | Palabras clave | Stem cell markers | es |
| dc.subject | Palabras clave | Transcriptomics | es |
| dc.title | Título | Purification and transcriptomic characterization of proliferative cells of Mesocestoides corti selectively affected by irradiation | es |
| dc.type | Tipo de documento | Artículo | es |
| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| 10.3389-fpara.2024.1362199.pdf | — | 10.32 MB | Adobe PDF |