Título:
Transcriptome-wide analysis of microRNA–mRNA correlations in tissue identifies microRNA targeting determinants
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Autor:
Trinidad Barnech, Juan Manuel
Fort Canobra, Rafael S
Trinidad Barnech, Guillermo
Garat, Beatriz
Duhagon, María Ana
Fort Canobra, Rafael S
Trinidad Barnech, Guillermo
Garat, Beatriz
Duhagon, María Ana
Tutor:
Tipo de documento:
Artículo
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Palabras clave:
MicroRNA
3' supplementary pairing
Transcriptome
TCGA
Offset
GC-content
Correlation
3' supplementary pairing
Transcriptome
TCGA
Offset
GC-content
Correlation
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Año de publicación:
2023
Contenido:
Resumen:
MicroRNAs are small RNAs that regulate gene expression through complementary base pairing with their target mRNAs. A substantial understanding of microRNA target recognition and repression mechanisms has been reached using diverse empirical and bioinformatic approaches, primarily in vitro biochemical or cell culture perturbation settings. We sought to determine if rules of microRNA target efficacy could be inferred from extensive gene expression data of human tissues. A transcriptome-wide assessment of all the microRNA–mRNA canonical interactions’ efficacy was performed using a normalized Spearman correlation (Z-score) between the abundance of the transcripts in the PRAD-TCGA dataset tissues (RNA-seq mRNAs and small RNA-seq for microRNAs,
546 samples). Using the Z-score of correlation as a surrogate marker of microRNA target efficacy, we confirmed hallmarks of microRNAs, such as repression of their targets, the hierarchy of preference for gene regions (3'UTR > CDS > 5'UTR), and seed length (6 mer < 7 mer < 8 mer), as well as the contribution of the 3'-supplementary pairing at nucleotides 13–16 of the microRNA. Interactions mediated by 6 mer + supplementary showed similar inferred repression as 7 mer sites, suggesting that the 6 mer + supplementary sites may be relevant in vivo. However, aggregated 7 mer-A1 seeds appear more repressive than 7 mer-m8 seeds, while similar when pairing possibilities at the 30 -supplementary sites. We then examined the 30-supplementary pairing using 39 microRNAs with Z-score-inferred repressive 3'-supplementary interactions. The approach was sensitive to the offset of the bridge between seed and 3'-supplementary pairing sites, and the pattern of offset-associated repression found supports previous findings. The 39 microRNAs with effective repressive 30 supplementary sites show low GC content at positions 13–16. Our study suggests that the transcriptome-wide analysis of microRNA–mRNA correlations may uncover hints of microRNA targeting determinants. Finally, we provide a bioinformatic tool to identify microRNA–mRNA candidate interactions based on the sequence complementarity of the seed and 3'
-supplementary regions.
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MDPI
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Non-Coding RNA, 2023, 9(1): 15.
Financiadores:
CSIC: I+D_2016_487
CSIC: I+D_2020_566
CSIC: I+D_2020_566
Citación:
Trinidad Barnech, J, Fort Canobra, R, Trinidad Barnech, G [y otros autores]. "Transcriptome-wide analysis of microRNA–mRNA correlations in tissue identifies microRNA targeting determinants". Non-Coding RNA. [en línea] 2023, 9: 15. 15 h. DOI: 10.3390/ncrna9010015.
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2311-553X
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Licencia Creative Commons Atribución (CC - By 4.0)
| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| 10.3390.ncrna9010015.pdf | — | 2.08 MB | Adobe PDF |