| Metadato | Etiqueta | Valor | Idioma |
|---|---|---|---|
| dc.contributor.author | Autor | Rodrigues Mendina, Graciana | - |
| dc.contributor.author | Autor | Brun, Victoria de | - |
| dc.contributor.author | Autor | Adrien Delgado, María de Lourdes | - |
| dc.contributor.author | Autor | Pons Romero, María Victoria | - |
| dc.contributor.author | Autor | Vivián Paradizo, Rodrigo Nicolás | - |
| dc.contributor.author | Autor | Gil, Jorge | - |
| dc.contributor.author | Autor | Rocha, Cecilia C. | - |
| dc.contributor.author | Autor | Binelli, Mario | - |
| dc.contributor.author | Autor | Meikle, Ana | - |
| dc.contributor.filiacion | Filiación | Rodrigues Mendina Graciana, Universidad de la República (Uruguay). Facultad de Veterinaria. Departamento de Ciencias Veterinarias y Agrarias Cenur Litoral Norte | - |
| dc.contributor.filiacion | Filiación | Brun Victoria de, Universidad de la República (Uruguay). Facultad de Veterinaria. Departamento de Clínicas y Hospital Veterinario | - |
| dc.contributor.filiacion | Filiación | Adrien Delgado María de Lourdes, Universidad de la República (Uruguay). Facultad de Veterinaria. Departamento de Ciencias Veterinarias y Agrarias Cenur Litoral Norte | - |
| dc.contributor.filiacion | Filiación | Pons Romero María Victoria, Universidad de la República (Uruguay). Facultad de Veterinaria. Departamento de Ciencias Veterinarias y Agrarias Cenur Litoral Norte | - |
| dc.contributor.filiacion | Filiación | Vivián Paradizo Rodrigo Nicolás, Universidad de la República (Uruguay). Facultad de Agronomía. Departamento de Producción Animal y Pasturas | - |
| dc.contributor.filiacion | Filiación | Gil Jorge, Universidad de la República (Uruguay). Facultad de Veterinaria. Departamento de Ciencias Veterinarias y Agrarias Cenur Litoral Norte | - |
| dc.contributor.filiacion | Filiación | Rocha Cecilia C., University of Florida (Gainesville, Florida). Department of Animal Sciences | - |
| dc.contributor.filiacion | Filiación | Binelli Mario, University of Florida (Gainesville, Florida). Department of Animal Sciences | - |
| dc.contributor.filiacion | Filiación | Meikle Ana, Universidad de la República (Uruguay). Facultad de Veterinaria. Departamento de Clínicas y Hospital Veterinario | - |
| dc.date.accessioned | Fecha ingreso | 2025-06-16T12:13:34Z | - |
| dc.date.available | Fecha disponible | 2025-06-16T12:13:34Z | - |
| dc.date.issued | Año de publicación | 2025 | - |
| dc.description.abstract | Resumen | In cattle, expression of IFN-stimulated genes in the female reproductive tract has been reported as an early pregnancy diagnostic tool, as early as d 17 of pregnancy. The hypothesis of this study was that expression of ISG15 in the cervix of pregnant heifers is increased on d 14 of pregnancy. The objective was to compare the expression of ISG15 in cervical cells between pregnant and cyclic heifers (control, sham-inseminated) on d 14, 16, and 18 after insemination (d 0). Holstein heifers were submitted to an estrus synchronization protocol and inseminated with extender only (“control,” n = 6), or with regular semen (n = 15). Heifers were classified as pregnant (n = 10) by ultrasound at d 30 through the detection of a viable embryo with a heartbeat. Blood samples from the coccygeal vein were collected to determine serum progesterone concentrations on d 14, 16, and 18. The expression of ISG15 and PGR in cervical cells collected through cytobrush was measured on d 14, 16, and 18. A receiver operating characteristic (ROC) curve was calculated to quantify the pregnancy diagnostic accuracy of ISG15 and PGR expression. The expression levels of ISG15 in cervical cells were significantly greater in pregnant compared with control heifers on d 14, and remained greater on d 16 and 18, whereas differences in PGR were observed only on d 18. Scatter plots and ROC analyses showed the most accurate prediction of pregnancy for ISG15 on d 16. In conclusion, cervical cells express greater ISG15 mRNA in pregnant versus control heifers as early as d 14 postinsemination, with the best accuracy on d 16. | es |
| dc.format.extent | Extensión | 7 h | es |
| dc.format.mimetype | Formato | application/pdf | es |
| dc.identifier.citation | Citación | Rodrigues Mendina, G, Brun, V, Adrien Delgado, M, Pons Romero, M, Vivián Paradizo, R, Gil, J, Rocha, C, Binelli, M y Meikle, A. Increased expression of interferon-stimulated gene 15 (ISG15) in cervical cells on day 14 of pregnancy in Holstein heifers. JDS Communications. [en línea] 2025 2025, 6(1), 165-170 | es |
| dc.identifier.doi | DOI | https://doi.org/10.3168/jdsc.2024-0596 | - |
| dc.identifier.uri | URI | https://hdl.handle.net/20.500.12008/50325 | - |
| dc.language.iso | Idioma | en | es |
| dc.relation.ispartof | EN | JDS Communications, 2025, 6(1), 165-170 | es |
| dc.rights | Derechos | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
| dc.rights.licence | Licencia | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) | es |
| dc.subject.other | Descriptores | BOVINOS | es |
| dc.subject.other | Descriptores | GESTACION | es |
| dc.subject.other | Descriptores | HOLSTEIN | es |
| dc.subject.other | Descriptores | INSEMINACION ARTIFICIAL | es |
| dc.title | Título | Increased expression of interferon-stimulated gene 15 (ISG15) in cervical cells on day 14 of pregnancy in Holstein heifers | es |
| dc.type | Tipo de documento | Artículo | es |
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| M10.pdf | — | 1.39 MB | Adobe PDF |