Por favor, use este identificador para citar o enlazar este ítem: https://hdl.handle.net/20.500.12008/55510

Título:

Akt Is S-Palmitoylated: A New Layer of Regulation for Akt

Otros títulos:

Coordinador:

Director:

Compilador:

Autor:

Blaustein, Matías
Piegari, Estefanía
Martínez Calejman, Camila
Vila, Antonella
Amante, Analía
Manese, María Victoria
Zeida, Ari
Abrami, Laurence
Veggetti, Mariela
Guertin, David A.
van der Goot, F. Gisou
Corvi, María Martha
Colman-Lerner, Alejandro

Tutor:

Tipo de documento:

Artículo

Editor:

Palabras clave:

Akt
S-palmitoylation
Autophagy
Cell differentiation
Cell signaling
Lysosomes
Subcellular localization
Golgi

Descriptores:

PROTEÍNA ONCOGÉNICA V-AKT
AUTOFAGIA
LIPOILACIÓN
TRANSDUCCIÓN DE SEÑAL
DIFERENCIACIÓN CELULAR
LISOSOMAS
FOSFORILACIÓN
PROTEÍNAS PROTO-ONCOGÉNICAS C-AKT

Año de publicación:

2021

Contenido:

Resumen:

The protein kinase Akt/PKB participates in a great variety of processes, including translation, cell proliferation and survival, as well as malignant transformation and viral infection. In the last few years, novel Akt posttranslational modifications have been found. However, how these modification patterns affect Akt subcellular localization, target specificity and, in general, function is not thoroughly understood. Here, we postulate and experimentally demonstrate by acyl-biotin exchange (ABE) assay and 3H-palmitate metabolic labeling that Akt is S-palmitoylated, a modification related to protein sorting throughout subcellular membranes. Mutating cysteine 344 into serine blocked Akt S-palmitoylation and diminished its phosphorylation at two key sites, T308 and T450. Particularly, we show that palmitoylation-deficient Akt increases its recruitment to cytoplasmic structures that colocalize with lysosomes, a process stimulated during autophagy. Finally, we found that cysteine 344 in Akt1 is important for proper its function, since Akt1-C344S was unable to support adipocyte cell differentiation in vitro. These results add an unexpected new layer to the already complex Akt molecular code, improving our understanding of cell decision-making mechanisms such as cell survival, differentiation and death.

Descripción:

metadata.articulos.dc.description.uri:

Editorial:

Frontiers Media

EN:

Frontiers in Cell and Developmental Biology. 2021;9

Financiadores:

Citación:

Blaustein M, Piegari E, Martínez Calejman C y otros. Akt Is S-Palmitoylated: A New Layer of Regulation for Akt. Frontiers in Cell and Developmental Biology [en línea]. 2021;9.16 p.

Citación:

metadata.articulos.cc.license.name:

ISBN:

e-ISBN:

ISSN:

ISMN:

Otros identificadores:

Cobertura geográfica:

Cobertura temporal:

Departamento académico:

Grupo de investigación:

Licencia:

Licencia Creative Commons Atribución (CC - By 4.0)

Colecciones:

Publicaciones Académicas y Científicas
FicherosDescripciónTamañoFormato
Akt Is S Palmitoylated.pdf Akt Is S Palmitoylated 10.69 MB Adobe PDF
Mostrar el registro Dublin Core completo del ítem