Título:
Computational analysis of a species D human adenovirus provides evidence of a novel virus
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Autor:
Espínola, Emilio E.
Barrios, Julio C.
Russomando, Graciela
Mirazo, Santiago
Arbiza, Juan
Barrios, Julio C.
Russomando, Graciela
Mirazo, Santiago
Arbiza, Juan
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Tipo de documento:
Artículo
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Palabras clave:
Adenovirus
Recombination
DNA sequencing
Bioinformatics
Recombination
DNA sequencing
Bioinformatics
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Año de publicación:
2017
Contenido:
Resumen:
A human adenovirus (HAdV) species D, was isolated from a hospitalised child with severe lower respiratory infection. It was initially detected in the nasopharyngeal aspirate of the child followed by conventional PCR amplification of the hexon, penton base, and fibre genes. Sanger DNA sequencing and phylogenetic analyses showed characteristics of a recombinant genome not described before. Next Generation Sequencing analysis was performed to reconstruct its complete DNA genome after viral isolation in adenocarcinoma human cell line (A549). A complete genomic sequence of 35.2 kb in length, with a G +C content of 57 % was obtained, related to HAdV-D29 (96 % identity). Imputed serology analysis demonstrated its novel type with a nucleotide sequence identity of 95.3 % (hexon loop 1) and 96 % (hexon loop 2) to HAdV-D9. The penton base gene showed a novel sequence, distantly related to HAdV-D44. The E3 and E4 regions evolved significantly from their ancestors. The fibre gene was almost identical to the knob region of HAdV-D15 but showed an unrelated shaft sequence. In conclusion the genomics of this novel HAdV, designated the HAdV-D83 [P83H9F15] prototype and bearing a new penton base gene, supports the importance of viral evolution to understand modified tissue tropism, nhanced transmission, or altered virulence.
Descripción:
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Microbiology Society
EN:
Journal of General Virology, 2017, 98: 2810–2820.
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Citación:
Espínola, E, Barrios, J, Russomando, G. [y otros autores]. "Computational analysis of a species D human adenovirus provides evidence of a novel virus". Journal of General Virology. [en línea] 2017, 98, 2810–2820. 11 h.
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0022-1317
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Licencia:
Licencia Creative Commons Atribución (CC - By 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| 10.1099jgv.0.000947.pdf | — | 1.25 MB | Adobe PDF |
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Excepto donde se indique lo contrario, la licencia de este ítem se describe como Licencia Creative Commons Atribución (CC - By 4.0)