Por favor, use este identificador para citar o enlazar este ítem: https://hdl.handle.net/20.500.12008/42622

Título:

Characterization of Leptospira isolates from humans and the environment in Uruguay

Otros títulos:

Coordinador:

Director:

Compilador:

Autor:

Meny, Paulina
Menéndez, Clara
Quintero, Jair
Hernández, Elba
Ríos, Cristina
Teruszkin Balassiano, Ilana
Nunes dos Reis Trindade, Camila
Magalhães Vital-Brazil, Juliana
Mendes Varela Ramos, Tatiane
Ashfield, Natalia
Feble, Camila
Avila, Esthefani
Schelotto, Felipe
Varela, Gustavo

Tutor:

Tipo de documento:

Artículo

Editor:

Palabras clave:

Leptospira
Human isolates
MLVA-VNTR-PCR
Leptospirosis
Environment Leptospira
Leptospira interrogans
Leptospira kirschneri
L. meyeri

Descriptores:

Año de publicación:

2017

Contenido:

Resumen:

Laboratory diagnosis of human leptospirosis usually relies on indirect methods exploring specific immune response. Isolation and identification of the involved strains are cumbersome, but can provide biological resources for pathogenic studies and relevant information for guiding prevention and control measures. The aim of the research we are hereby reporting was the characterization of Leptospira isolates obtained from humans and the environment in Uruguay. Blood cultures were performed from early samples of 302 Uruguayan patients, mainly rural workers, and from 36 water samples taken from their living or working environments. Eight human isolates and seven environmental isolates were obtained and analyzed by end point Polymerase Chain Reaction (PCR), Multilocus Variable Number of Tandem Repeat Analysis (MLVA) and other molecular methods. Human isolates corresponded to several serogroups and serovars of Leptospira interrogans and Leptospira kirschneri species, probably reflecting the infection with similar involved Leptospira species and serovars of an extended animal reservoir in rural settings of the country, mostly dedicated to meat and dairy production. Culture-positive patients were older than usually affected workers, and presented signs and symptoms of severe illness. A high organic and circulating bacterial burden may explain an easier positive result from these workers’ samples. Environmental isolates were mainly identified as Leptospira biflexa strains, with a single L. meyeri isolate of uncertain significance.

Descripción:

metadata.articulos.dc.description.uri:

Editorial:

EN:

Rev Inst Med Trop São Paulo. 59, 2017

Financiadores:

Citación:

MENY, P., MENENDEZ, C., QUINTERO, J., y otros. Characterization of Leptospira isolates from humans and the environment in Uruguay. Rev Inst Med Trop São Paulo [en línea] 59, 2017. Doi: 10.1590/S1678-9946201759079

Citación:

metadata.articulos.cc.license.name:

ISBN:

e-ISBN:

ISSN:

ISMN:

Otros identificadores:

Cobertura geográfica:

Cobertura temporal:

Departamento académico:

Grupo de investigación:

Licencia:

Licencia Creative Commons Atribución (CC - By 4.0)

Colecciones:

Artículos
FicherosDescripciónTamañoFormato
Characterization of Leptospira isolates from humans and the environment in Uruguay.pdf Artículo original 355.37 KB Adobe PDF
Mostrar el registro Dublin Core completo del ítem