Título:
Development, characterization, and evaluation of chi-Tn mAb-functionalized DOTAP-PLGA hybrid nanoparticles loaded with docetaxel for lung cancer therapy
Otros títulos:
Coordinador:
Director:
Compilador:
Autor:
Castro, Analía
Pittini, Álvaro
Berois, Nora
Faccio, Ricardo
Miranda, Pablo
Mombrú, Álvaro W.
Osinaga, Eduardo
Pardo, Helena
Pittini, Álvaro
Berois, Nora
Faccio, Ricardo
Miranda, Pablo
Mombrú, Álvaro W.
Osinaga, Eduardo
Pardo, Helena
Tutor:
Tipo de documento:
Artículo
Editor:
Palabras clave:
PLGA
DOTAP
Nanotecnología
Anticuerpo chi-tn
Lípidos
Polímeros
Cáncer
Antígeno TN
DOTAP
Nanotecnología
Anticuerpo chi-tn
Lípidos
Polímeros
Cáncer
Antígeno TN
Descriptores:
Año de publicación:
2025
Contenido:
Resumen:
Background/Objectives: The focus of this study was to prepare and characterize
docetaxel (DCX)-loaded lipid/polymer hybrid nanoparticles (LPHNps) functionalized with
the monoclonal antibody (mAb) Chi-Tn for a potential active targeting approach in lung
cancer treatment. Methods: We synthesized DOTAP-PLGA hybrid nanoparticles loaded
with DCX and functionalized them with Chi-Tn mAb through a biotin–avidin approach.
The physicochemical characterization involved dynamic light scattering, transmission
electron microscopy, Raman spectroscopy, and atomic force microscopy. The in vitro and
in vivo evaluations encompassed uptake studies, cell viability tests, and the assessment
of tumor growth control in a lung cancer model. Results: The nanoparticles featured
a hydrophobic PLGA core with 99.9% DCX encapsulation efficiency, surrounded by a
DOTAP lipid shell ensuring colloidal stability with a high positive surface charge. The
incorporation of PEGylated lipids on their surface helps evade the immune system and
facilitate Chi-Tn mAb attachment. The resulting nanoparticles exhibit a spherical shape
with monodisperse particle sizes averaging 250 nm, and demonstrate sustained drug
release. In vitro uptake studies and viability assays conducted in A549 cancer cells show
that the Chi-Tn mAb enhances nanoparticle internalization and significantly reduces cell
viability. In vivo studies demonstrate a notable reduction in tumor volume and an increased
survival rate in the A549 tumor xenograft mice model when DCX was encapsulated in
nanoparticles and targeted with Chi-Tn mAb in comparison to the free drug. Conclusions:
Therefore, Chi-Tn-functionalized LPHNps hold promise as carriers for actively targeting
DCX to Tn-expressing carcinomas.
Descripción:
metadata.articulos.dc.description.uri:
Editorial:
MDPI
EN:
Pharmaceutics, v.17, n°2, 2025. -- e164
Financiadores:
Citación:
Castro, A., Pittini, Á., Berois, N. y otros. "Development, characterization, and evaluation of chi-Tn mAb-functionalized DOTAP-PLGA hybrid nanoparticles loaded with docetaxel for lung cancer therapy". Pharmaceutics [en línea], v.17, n°2, 2025. -- e164. 16 p.
Citación:
metadata.articulos.cc.license.name:
ISBN:
e-ISBN:
ISSN:
ISMN:
Otros identificadores:
Cobertura geográfica:
Cobertura temporal:
Departamento académico:
Grupo de investigación:
Licencia:
Licencia Creative Commons Atribución (CC - By 4.0)
Colecciones:
| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| Development, characterization.pdf | — | 2.49 MB | Adobe PDF |