Título:
Hypoxia, acidification and oxidative stress in cells cultured at large distances from an oxygen source
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Autor:
D’Aiuto, Natali
Hochmann Valls, Jimena
Millán Santarcieri, Magdalena
Di Paolo, Andrés
Sotelo-Silveira, José Roberto
Arocena, Miguel
Bologna-Molina, Ronell
Hochmann Valls, Jimena
Millán Santarcieri, Magdalena
Di Paolo, Andrés
Sotelo-Silveira, José Roberto
Arocena, Miguel
Bologna-Molina, Ronell
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Tipo de documento:
Artículo
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Palabras clave:
Biochemistry
Cancer
Cell biology
Cancer
Cell biology
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Año de publicación:
2022
Contenido:
Resumen:
Hypoxia is a condition frequently encountered by cells in tissues, whether as a normal feature of their microenvironment or subsequent to deregulated growth. Hypoxia can lead to acidification and increased oxidative stress, with profound consequences for cell physiology and tumorigenesis. Therefore, the interplay between hypoxia and oxidative stress is an important aspect for understanding the effects of hypoxic microenvironments on cells. We have used a previously developed variant of the method of coverslip‑induced hypoxia to study the process of acidification in a hypoxic microenvironment and to simultaneously visualize intracellular levels of hypoxia and oxidative stress. We observed high accumulation of CO 2 in hypoxic conditions, which we show is the main contributor to acidification in our model. Also, increased levels of oxidative stress were observed in moderately hypoxic cells close to the oxygen source, where the mitochondrial membrane potential was preserved. Conversely, cells at large distances from the oxygen source showed higher
levels of hypoxia, milder oxidative stress and reduced mitochondrial membrane potential. Our results contribute to characterize the interplay between reduced oxygen levels, acidification and oxidative stress in a simple in vitro setting, which can be used to model cell responses to an altered environment, such as the early tumor microenvironment.
Descripción:
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Editorial:
Springer Nature
EN:
Scientific Reports, 2022, 12: 21699.
Financiadores:
CSIC, PEDECIBA y ANII.
Citación:
D’Aiuto, N, Hochmann Valls, J, Millán Santarcieri, M, [y otros autores]. "Hypoxia, acidification and oxidative stress in cells cultured at large distances from an oxygen source". Scientific Reports. [en línea] 2022, 12: 21699. 9 h. DOI: 10.1038/s41598-022-26205-y
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ISSN:
2045-2322
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Licencia:
Licencia Creative Commons Atribución (CC - By 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| 101038s4159802226205y.pdf | — | 1.52 MB | Adobe PDF |