| Metadato | Etiqueta | Valor | Idioma |
|---|---|---|---|
| dc.contributor.author | Autor | Martinez-Rosales, Cecilia | - |
| dc.contributor.author | Autor | Marizcurrena, Juan José | - |
| dc.contributor.author | Autor | Iriarte, Andrés | - |
| dc.contributor.author | Autor | Fullana, Natalia | - |
| dc.contributor.author | Autor | Musto, Héctor | - |
| dc.contributor.author | Autor | Castro-Sowinski, Susana | - |
| dc.contributor.filiacion | Filiación | Martinez-Rosales Cecilia, Universidad de la República (Uruguay). Facultad de Ciencias. Sección Bioquímica y Biología Molecular | - |
| dc.contributor.filiacion | Filiación | Marizcurrena Juan José, Universidad de la República (Uruguay). Facultad de Ciencias. Sección Bioquímica y Biología Molecular | - |
| dc.contributor.filiacion | Filiación | Iriarte Andrés, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Desarrollo Biotecnológico | - |
| dc.contributor.filiacion | Filiación | Fullana Natalia, Universidad de la República (Uruguay). Facultad de Ciencias. Sección Bioquímica y Biología Molecular | - |
| dc.contributor.filiacion | Filiación | Musto Héctor, Universidad de la República (Uruguay). Facultad de Ciencias. Laboratorio de Organización y Evolución del Genoma | - |
| dc.contributor.filiacion | Filiación | Castro-Sowinski Susana, Universidad de la República (Uruguay). Facultad de Ciencias. Sección Bioquímica y Biología Molecular | - |
| dc.date.accessioned | Fecha ingreso | 2026-07-09T16:07:44Z | - |
| dc.date.available | Fecha disponible | 2026-07-09T16:07:44Z | - |
| dc.date.issued | Año de publicación | 2015 | - |
| dc.description.abstract | Resumen | We report the isolation of a cold-adapted bacterium belonging to the genus Janthinobacterium (named AU11), from a water sample collected in Lake Uruguay (King George Island, South Shetlands). AU11 (growth between 4°C and 30°C) produces a single cold-active extracellular protease (ExPAU11), differentially expressed at low temperature. ExPAU11 was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS) as an alkaline metallo-protease (70% coverage with an extracellular protease of Janthinobacterium sp. PI12), and by protease-inhibitor screening identified as a serine-protease. To the best of our knowledge this is the first experimental evidence of a cold-active extracellular protease produced by Janthinobacterium. Furthermore, we identified a serine-protease gene (named JSP8A) showing 60% identity (98% query coverage) to subtilisin peptidases belonging to the S8 family (S8A subfamily) of many cyanobacteria. A phylogenetic analysis of the JSP8A protease, along with related bacterial protein sequences, confirms that JSP8A clusters with S8A subtilisin sequences from different cyanobacteria, and is clearly separated from S8A bacterial sequences of other phyla (including its own). An analysis of the genomic organization around JSP8A suggests that this protease gene was acquired in an event that duplicated a racemase gene involved in transforming L- to D-amino acids. Our results suggest that AU11 probably acquired this subtilisin-like protease gene by horizontal gene transfer (HGT) from a cyanobacterium. We discuss the relevance of a bacterial protease-HGT in the Antarctic environment in light of this hypothesis. | es |
| dc.description.sponsorship | Financiadores | Programa de Desarrollo de las Ciencias Básicas (PEDEClBA) | es |
| dc.description.sponsorship | Financiadores | Instituto Antártico Uruguayo (IAU) | es |
| dc.description.sponsorship | Financiadores | Agencia Nacional de Investigación e Innovación (ANII) | es |
| dc.format.mimetype | Formato | application/pdf | es |
| dc.identifier.citation | Citación | MARTINEZ-ROSALES, C., MARIZCURRENA, JJ., IRIARTE, A., y otros. Characterizing proteases in an Antarctic Janthinobacterium sp. isolate: Evidence of a protease horizontal gene transfer event. Adv Polar Sci [en línea] 2015, 26(1). DOI: 10.13679/j.advps.2015.1.00088 | es |
| dc.identifier.doi | DOI | 10.13679/j.advps.2015.1.00088 | - |
| dc.identifier.uri | URI | https://hdl.handle.net/20.500.12008/55977 | - |
| dc.language.iso | Idioma | en | es |
| dc.relation.ispartof | EN | Adv Polar Sci. 26(1), 2015 | es |
| dc.rights | Derechos | Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014) | es |
| dc.rights.licence | Licencia | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) | es |
| dc.subject | Palabras clave | Antarctic | es |
| dc.subject | Palabras clave | cold-active protease | es |
| dc.subject | Palabras clave | horizontal gene transfer | es |
| dc.subject | Palabras clave | Janthinobacterium | es |
| dc.subject | Palabras clave | subtilisin | es |
| dc.title | Título | Characterizing proteases in an Antarctic Janthinobacterium sp. isolate: Evidence of a protease horizontal gene transfer event | es |
| dc.type | Tipo de documento | Artículo | es |
Colecciones:
| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| Characterizing proteases in an Antarctic Janthinobacterium.pdf | Artículo original | 1.91 MB | Adobe PDF |