Título:
Outbreak of Pseudomonas aeruginosa High-Risk Clone ST309 Serotype O11 Featuring blaPER-1 and qnrVC6
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Autor:
Papa-Ezdra, Romina
Outeda, Matilde
Cordeiro, Nicolás F.
Araújo, Lucía
Gadea, Pilar
García-Fulgueiras, Virginia
Seija, Verónica
Bado, Inés
Vignoli, Rafael
Outeda, Matilde
Cordeiro, Nicolás F.
Araújo, Lucía
Gadea, Pilar
García-Fulgueiras, Virginia
Seija, Verónica
Bado, Inés
Vignoli, Rafael
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Palabras clave:
Pseudomonas aeruginosa
ESBL
blaPER-1
Transposon
ST309
ESBL
blaPER-1
Transposon
ST309
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Año de publicación:
2024
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Resumen:
Pseudomonas aeruginosa is a leading cause of hospital-acquired infections worldwide. Biofilm production, antibiotic resistance, and a wide range of virulence factors contribute to their persistence in nosocomial environments. We describe an outbreak caused by a multidrug-resistant P. aeruginosa strain in an ICU. Antibiotic susceptibility was determined and blaPER-1 and qnrVC were amplified via PCR. Clonality was determined using PFGE and biofilm formation was studied with a static model. A combination of antibiotics was assessed on both planktonic cells and biofilms. WGS was performed on five isolates. All isolates were clonally related, resistant to ceftazidime, cefepime, amikacin, and ceftolozane-tazobactam, and harbored blaPER-1; 11/19 possessed qnrVC. Meropenem and ciprofloxacin reduced the biofilm biomass; however, the response to antibiotic combinations with rifampicin was different between planktonic cells and biofilms. WGS revealed that the isolates belonged to ST309 and serotype O11. blaPER-1 and qnrVC6 were associated with a tandem of ISCR1 as part of a complex class one integron, with aac(6′ )-Il and ltrA as gene cassettes. The structure was associated upstream and downstream with Tn4662 and flanked by direct repeats, suggesting its horizontal mobilization capability as a composite transposon. ST309 is considered an emerging high-risk clone that should be monitored in the Americas.
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Antibiotics. 13(2) 159, 2024
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PAPA-EZDRA, R., OUTEDA, M., CORDEIRO, NF., y otros. Outbreak of Pseudomonas aeruginosa High-Risk Clone ST309 Serotype O11 Featuring blaPER-1 and qnrVC6. Antibiotics [en línea] 2024, 13(2) 159. DOI: 10.3390/antibiotics13020159
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Licencia Creative Commons Atribución (CC - By 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| Outbreak of Pseudomonas aeruginosa High-Risk Clone ST309 Serotype O11 Featuring blaPER-1 and qnrVC6.pdf | Artículo original | 2.2 MB | Adobe PDF |