Título:
Xylosaccharides purification from eucalyptus residues for L-lactic acid production by Weizmannia coagulans.
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Autor:
Cabrera, María-Noel
Vila, Eugenia
Liguori, Alberto
D ́Andrada, Constanza
Moure, Santiago
Guigou, Mairan
Cebreiros, Florencia
Rodao, Juan Martín
Camesasca, Laura
Ferrari, Mario Daniel
Lareo, Claudia
Vila, Eugenia
Liguori, Alberto
D ́Andrada, Constanza
Moure, Santiago
Guigou, Mairan
Cebreiros, Florencia
Rodao, Juan Martín
Camesasca, Laura
Ferrari, Mario Daniel
Lareo, Claudia
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Tipo de documento:
Preprint
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Palabras clave:
Xylosaccharides purification
Adsorptive and anionics resin
L-lactic acid
W. coagulans
lignocellulosic residue
Adsorptive and anionics resin
L-lactic acid
W. coagulans
lignocellulosic residue
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Año de publicación:
2024
Contenido:
Resumen:
In this work, phosphoric acid pretreatment (0.6% H3PO4, 160°C, 40 min) of
eucalyptus residues was performed to recover the hemicellulosic fraction for further
conversion to L-lactic acid by fermentation with Weizmannia coagulans DSM 2314
(formerly Bacillus coagulans). The hemicellulosic hydrolysate was composed of
xylosaccharides 24.8 g/L (mainly xylose, 20.3 g/L), acetic acid 7.8 g/L, furfural 0.7 g/L,
and acid soluble lignin (ASL) 2.1 g/L. It was subsequently purified by using anion
exchange or adsorption resins. Different liquor-to-resin ratios were evaluated to obtain a
high concentration of xylosaccharides in the eluate and high removal of components
that inhibit lactic fermentation. The best performance was observed when using
Amberlite®-XAD-4 resin at a liquor-to-resin ratio of 3:1. An eluted hydrolysate was
obtained, preserving 80% of the xylosaccharides and effectively removing almost all
furfural, 90% of ASL, and 32% of acetic acid. Subsequently, L-lactic acid production
by W. coagulans DSM 2314 was evaluated using concentrated non-detoxified and detoxified hydrolysate obtained through vacuum oven. For the non-detoxified
hydrolysate, 12 g/L of L-lactic acid was obtained after 48 h showing a yield of 0.56 g
lactic acid/g sugar and a xylose consumption of 62%. The detoxification of the liquor
significantly improved the fermentation performance of W. coagulans, resulting in a
concentration of 16 g/L of lactic acid after 24 h, with a yield of 0.73 glactic acid/gsugar, and
almost complete xylose consumption.
Descripción:
El artículo fue publicado en la revista Biofuels, Bioproducts and Biorefining, con el título Purification of xylosaccharides from eucalyptus residues for l-lactic acid production by Weizmannia coagulans.
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Biofuels, Bioproducts and Biorefining, July 2024.
Financiadores:
Proyecto ANII FSE_1_2019_1_159574, Producción integrada de etanol de segunda generación, ácido láctico y coproductos a partir de desechos forestales bajo un enfoque de biorrefinería.
Citación:
Cabrera, M., Vila, E., Liguori, A. y otros. Xylosaccharides purification from eucalyptus residues for L-lactic acid production by Weizmannia coagulans. [Preprint] Publicado en: Boifuels, Bioproducts and Biorefining, July 2024. 31 p. DOI: 10.1002/bbb.2662.
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Licencia:
Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| CVLDMGCRCFL24.pdf | — | 834.16 KB | Adobe PDF |