Título:
Whole-genome sequencing analysis from the Chikungunya Virus Caribbean outbreak reveals novel evolutionary genomic elements
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Autor:
Stapleford, K.
Moratorio, Gonzalo
Henningsson, R.
Chen, R.
Matheus, S.
Enfissi, A.
Weissglas-Volkov, D.
Isakov, O.
Blanc, H.
Mounce, B. C.
Dupont-Rouzeyrol, M.
Shomron, N.
Weaver, S.
Fontes, Magnus
Rousset, D.
Vignuzzi, Marco
Moratorio, Gonzalo
Henningsson, R.
Chen, R.
Matheus, S.
Enfissi, A.
Weissglas-Volkov, D.
Isakov, O.
Blanc, H.
Mounce, B. C.
Dupont-Rouzeyrol, M.
Shomron, N.
Weaver, S.
Fontes, Magnus
Rousset, D.
Vignuzzi, Marco
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Tipo de documento:
Artículo
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Palabras clave:
Chikungunya
Togaviridae
Genética
Virus
Togaviridae
Genética
Virus
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Año de publicación:
2016
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Resumen:
Background Chikungunya virus (CHIKV), an alphavirus and member of the Togaviridae family, is capableof causing severe febrile disease in humans. In December of 2013 the Asian Lineage of CHIKV spread from the Old World to the Americas, spreading rapidly throughout the New World. Given this new emergence in naïve populations we studied the viral genetic diversity present in infected individuals to understand how CHIKV may have evolved during this continuing outbreak. Methodology/Principle Findings We used deep-sequencing technologies coupled with well-established bioinformatics pipelines to characterize the minority variants and diversity present in CHIKV infected individuals from Guadeloupe and Martinique, two islands in the center of the epidemic. We observed changes in the consensus sequence as well as a diverse range of minority variants present at various levels in the population. Furthermore, we found that overall diversity was dramatically reduced after single passages in cell lines. Finally, we constructed an infectious clone from this outbreak and identified a novel 3’ untranslated region (UTR) structure, not previously found in nature, that led to increased replication in insect cells. Conclusions/Significance Here we preformed an intrahost quasispecies analysis of the new CHIKV outbreak in the Caribbean. We identified novel variants present in infected individuals, as well as a new 3’UTR structure, suggesting that CHIKV has rapidly evolved in a short period of time once it entered this naïve population. These studies highlight the need to continue viral diversity surveillance over time as this epidemic evolves in order to understand the evolutionary potential of CHIKV.
Descripción:
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PLoS
EN:
PLoS Neglected Tropical Diseases, 2016 10(1): e0004402
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Citación:
STAPLEFORD, K.A., y otros."Whole-genome sequencing analysis from the Chikungunya Virus Caribbean outbreak reveals novel evolutionary genomic elements" PLoS Neglected Tropical Diseases [en línea]. 2016 10(1): e0004402. doi: 10.1371/journal.pntd.0004402
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1935-2735
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Licencia Creative Commons Atribución (CC - By 4.0)
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| Ficheros | Descripción | Tamaño | Formato | ||
|---|---|---|---|---|---|
| 101371journalpntd0004402.pdf | — | 1.86 MB | Adobe PDF |